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The public health importance of Kudoa infection in fish remains unclear. Recently in Japan a Kudoa species, K. septempunctata, was newly implicated as a causative agent of unidentified food poisoning related to the consumption of raw olive flounder. Other marine fishery products are also suspected as causative raw foods of unidentified food poisoning. For this study, we detected kudoid parasites from sliced raw muscle tissues of a young Pacific bluefin and an adult yellowfin tuna. No cyst or pseudocyst was evident in muscles macroscopically, but pseudocysts were detected in both samples histologically. One substitution (within 1100 bp overlap) and ten substitutions (within 753 bp overlap) were found respectively between the partial sequences of 18S and 28S rDNAs from both isolates. Nucleotide sequence similarity searching of 18S and 28S rDNAs from both isolates showed the highest identity with those of K. neothunni from tuna. Based on the spore morphology, the mode of parasitism, and the nucleotide sequence similarity, these isolates from a Pacific bluefin and a yellowfin tuna were identified as K. neothunni. Phylogenetic analysis of the 28S rDNA sequence revealed that K. neothunni is classifiable into two genotypes: one from Pacific bluefin and the other from yellowfin tuna. Recently, an unidentified kudoid parasite morphologically and genetically similar K. neothunni were detected from stocked tuna samples in unidentified food poisoning cases in Japan. The possibility exists that K. neothunni, especially from the Pacific bluefin tuna, causes food poisoning, as does K. septempunctata.
Background. Bigeye tuna, Thunnus obesus (Lowe, 1839); yellowfin tuna, Thunnus albacares (Bonnaterre, 1788); and albacore, Thunnus alalunga (Bonnaterre, 1788), are very important species for world fisheries. It is crucial to the future existence of those economically important species that the best possible biological data on the species is provided to fisheries managers. Materials and Methods. The weight–length relations (WLRs) for bigeye tuna, yellowfin tuna, and albacore, collected in the Atlantic, Indian, and eastern Pacific oceans were studied using commonly accepted methodology. Results. Significant differences can be found from the fork length distributions and the WLRs of the above 3 tuna species and the relations of gilled-gutted and whole weight of bigeye and yellowfin tunas collected from the Atlantic, Indian, and Eastern Pacific Oceans. Significant differences of fork length distributions can be found for bigeye tuna, yellowfin tuna, and albacore from the three areas. The growth exponents (b) of bigeye tuna, yellowfin tuna, and albacore collected from the Atlantic, Indian, and eastern Pacific oceans register significant deviations from isometric value of 3. Conclusion. The date collected will be useful for the fisheries management of the three species studied.
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