The purpose of this study was to determine the effect of long-term exercise of varying intensity on selected hematological blood parameters of horses. The study was conducted on 24 clinically healthy half-blood horses. Blood for designations was collected twice: at the beginning of March, when the indoor season was approaching an end (a smaller number of starts), and at the end of May, when efforts were particularly intensified due to the continuous training and the beginning of the open season (more starts). The whole blood was examined for: the total number of erythrocytes (RBC), hematocrit (Ht), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and the red blood cell distribution width (RDW). The study was performed using an MS9 hematology analyzer. Mean values were compared between the retrievals (stallions and mares), and animals of both sexes were compared among themselves in collections I and II. The study showed that the growing physical effort of sport horses caused increased rates of hematological parameters, such as RBC, Ht, MCV, RDW in both stallions and mares in collection II compared to collection I. Studies of hematological parameters are an important element of planning and monitoring the workouts of horses.
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