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The objective of the studies was an epidemic analysis of Feline leukemia virus (FeLV) infections in various habitats and the evaluation of the results of diagnostic examinations of 1263 samples of blood serum, 187 samples of saliva and 187 samples of tears by the use of ELISA test. Comparative serological examinations of the presence of FeLV in serum, saliva and tears were done on 187 animals by the use of the ELISA kits (dr. Lutz, Zurich, Switzerland). Comparative examinations of the presence of FeLV were performed with the use of three different diagnostic ELISA kits on 67 animals. A mean idex of infection in a group of clinically normal cats was 3.8%, in cats living in groups 14.6%, while in cats showing various clinical symptoms 17.6%. Phyletic cats were almost twice as rarely infected than mongrels in a free-house system. The highest percentage of FeLV infections (17.8%) was found in cats living in groups at the age of less than 1 year and more than 8 years (17.1%), the lowest percentage of FeLV infection (11.4%) was noted in cats at 2, 3 and 5 years of age. Sex did not affect the percentage of infections, although FeLV infections were found twice as often in noncastrated (70.6%) than in castrated cats (29.4%). In females sterilization did not influence the percentage of FeLV infection. Estimating as 100% the number of positive results obtained with blood samples, 81.3% samples of saliva and 75.0% samples of tears were seropositive. These results point to the usefulness of saliva and tear samples for diagnostic tests. The noted full agreement of positive and negative results obtained in three compared diagnosis ELISA kits points to the sensitivity and specificity of the tests used.
One hundred and twenty three sera of cats were tested by the ELISA method for the presence of antibodies against FeLV antigen. The prevalence rate of infection was 12.1%. The infection was found in clinically ill cats usually over two years of age. There was no significant difference between sexes found in FeLV positive animals. In the group of healthy cats and cats aged up to eight weeks, no antibodies against p. 27 antigen of FeLV were discovered. Besides, it was noted that four of the FeLV positive cats (4 out of 15) were also infected with Hemobartonella felis. Haematological survey revealed that these animals suffered from a more severe anaemia than those infected with H. felis of FeLV alone.
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