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The aim of this study was to determine the composition of nitrogen fractions in alfalfa and red clover, which differ in proteolytic activity, and to evaluate the effect of wilting on changes in nitrogen fractions in alfalfa and red clover herbage. Total nitrogen was divided into protein and non-protein nitrogen, and the amino acid profile of protein was analyzed. Buffer-soluble nitrogen (BSN), including buffer-soluble protein nitrogen (BSPN) and non-protein buffer-soluble nitrogen (NPBSN), was determined. The NPBSN fraction was further subdivided into peptide nitrogen, amino acid nitrogen, neutral detergent-insoluble nitrogen (NDIN) and acid detergent-insoluble nitrogen (ADIN). Wilting in the field to 40% dry matter content (the swath was tedded once) did not reduce the total nitrogen content of alfalfa and red clover herbage, although it affected the concentration of the BSNfraction, in particular NPBSN, and free amino acid nitrogen. During alfalfa wilting, the soluble protein content decreased and the concentrations of non-protein nitrogen compounds increased, mostly due to an increase in free amino acid nitrogen. A reverse trend was observed during red clover wilting – the concentrations of non-protein nitrogen compounds decreased and soluble protein content increased. A decrease was also noted in peptide nitrogen, NDIN and ADIN. Wilting of alfalfa and red clover had no adverse effect on the amino acid profile of protein. The concentrations of essential amino acids that limit milk protein synthesis, i.e. Lys, Met, His and Arg, did not decrease. Legume wilting in the field contributes to an increase in the concentrations of soluble nitrogen in the plant material intended for ensiling.
Fusarium spp. causes yellowing, corm rot, browning of foliage and wilting in gladiolus. It reduces the quality, yield and market value of gladiolus. This disease is caused by the Fusarium species; namely Fusarium oxysporum f. sp. gladioli, F. solani, F. moniliforme and F. roseum in gladiolus. F. oxysporum f. sp. gladioli (Massay) Snyder and Hansen is the most common and worldwide in distribution. The fungus can survive in soil indefinitely as mycelium, clamydospores, microconidia and macroconidia. Infected corms show tissue discoloration. The corms become softened, wrinkled and mummified in storage. Despite many attempts to control this disease, the problem is still important worldwide. The management practices generally em­ployed for its control include resistant cultivars, chemical applications, cultural prac­tices and biotechnological approaches. However, incorporation of integrated man­agement provides a better opportunity to manage this disease. In this review, the re­ports on the major progress made in management of Fusarium yellows in gladiolus species have been discussed.
In five monitored farms, stem base rot symptoms and carnation wilting were observed on 10–35% of plants and Rhizoctonia solani was the most frequently isolated species from diseased tissues. Additionally Fusarium avenaceum and Botrytis cinerea were only rarely noticed in the diseased stem parts. Isolates from carnation and eustoma caused stem and leaf blade rot of carnation but tissues were colonized significantly faster by a culture from a host plant. Different reaction of three carnation cultivars was observed on R. solani inoculation. The optimal species growth was observed at 30°C and it was also the most favorable temperature for colonization of carnation stem parts. In the greenhouse trial isolate of R. solani obtained from carnation crops caused stem base rot already after 2-week-growth on 1 per 5 plant in each replication and within the next 2 weeks 4 per 5 of plants died.
Phytophthora cryptogea izolowano z większości badanych roślin Alstroemeria xhybrida z objawami zgnilizny korzeni i kłączy. W testach laboratoryjnych gatunek ten kolonizował liście i fragmenty łodyg 8 odmian alstremerii, przy czym nekroza rozwijała się najszybciej na odm. ’Lorena’, a najwolniej na odm. ‘Rebecca’. Izolaty P. cryptogea z 5 różnych roślin gospodarzy kolonizowały liście i fragmenty łodyg 6 odmian alstremerii. Uzyskane wyniki wskazują na istotne różnice w ich patogeniczności w stosunku do poszczególnych badanych odmian.
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