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We used different tests to assess the effect of high soil concentrations of heavy metals on pollen viability in plants from metallicolous (MET) and nonmetallicolous (NONMET) populations. The frequency of viable pollen depended on the test applied: MET plants showed no significant reduction of pollen viability by acetocarmine, Alexander, MTT and X-Gal dye testing, but a drastic reduction of pollen viability in MET flowers (MET 56% vs 72% NONMET) by the FDA test. There was no correlation between pollen viability estimated in histochemical tests and pollen germination in vitro or in vivo. We discuss the terminology used to describe pollen viability as determined by histochemical tests.
The qualitative and quantitative analysis of polycyclic aromatic hydrocarbons contamination in selected popular brands of fruit and herbal teas was performed using GC-MS method. Among all teas similar quality profiles of PAHs were observed. In all determined compounds 4 light PAHs (phenanthrene, anthracene, fluoranthene, pyrene) from the group of PAHs listed by EPA were predominant. From group of 15 PAHs, marked out for analysis by The UE Scientific Committee on Food, only benzo[a]anthracene, chrysene, 5-metylchrysene, benzo[b]fluoranthene and benzo[k]fluoranthene were stated, although, their levels were relatively low. Benzo[a]pyrene and the most carcinogenic PAHs, namely dibenzopyrenes, were not detected. Moreover, the GC-MS analysis revealed significant differences in contamination level of particular brands of teas among all fruit and herbal teas under investigation.
The main aim of the study was to evaluate the antioxidant activity and total polyphenols content in fifteen herbal plants, such as chamomile flower head, oak bark, St. John’s-wort herb, hawthorn flower, dog rose and elder fruits, lingonberry leaf, and many more. From the herbs both the water and methanolic extracts were prepared. The highest antioxidant activity and the highest polyphenols level were obtained for lingonberry leaves (1752.9 mg Trolox/g d.w. and 199.6 mg catechin/g d.w., respectively). The oak bark, St. John’s-wort herb, and flowers of hawthorn and elder were also a very valuable source of antioxidant compounds. It was demonstrated that, in contrary to water, methanol was more efficient solvent for extraction of antioxidant compounds from lingonberry leaf, lime flower, fruits of elder and doge rose, oak bark, flowers of hawthorn, St. John’s-wort herb and heartsease herb. Therapeutic activity of some examined herbs did not depend on antioxidant activity.
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