Municipal solid waste landfills are considered to be important sources of groundwater contamination due to the leakage of leachate. Landfill leachate is undoubtedly one of the most challenging wastewaters in terms of treatment. Fungi can be used to treat a landfill leachate. Therefore, the aims of this research were to evaluate the biosorption potential of Pleurotus ostreatus as low-cost adsorbent for the toxicity removal from raw landfill leachate. The objective was also to study the change of leachate toxicity before and after biosorption tests using Sinapis alba L. growth inhibition test. It can be concluded that the growth inhibition (%) of Sinapis alba L. for landfill leachate samples after biosorption tests were in the range of 31.55–96.16%. These samples were strongly toxic, but the toxicity compared to samples before biosorption tests decreased for all samples.