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1

Adenosine deaminase in the diagnosis of white muscle diseases in lambs

100%
Altug N., Agaoglu Z. T., Yuksek N., Kaya A., Keles I.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 09
1007-1010
The aim of the study was to investigate the importance of ADA serum and its isoenzyme activity in diagnosing white muscle disease in lambs. The animal material used in the study was forty seven Akkaraman lambs - twenty seven of which had clinical signs of white muscle disease (white muscle disease group) and twenty healthy lambs (control group). Blood samples were taken and Se levels, CK, LDH, AST, ADA and ADA izoenzyme activity were determined. Following this, the animals in the white muscle disease group received 1 mg sodium selenit + 60 mg vitamin E intramuscularly as a single dose. Fourteen days following sodyum selenit + vitamin E application blood samples were again taken and the same analysis repeated. Apart from ADA2 activity, other enzyme activities were significantly high (p<0.001), and serum Se concentrations were significantly low (p<0.001) in animals of the white muscle disease group compared to the control group. When the parameters obtained from the white muscle disease group were compared before and after treatment it was indicated that all enzyme parameters decreased significantly (p<0.001) after treatment. On the other hand, serum Se concentration increased (p<0.01) after treatment in the diseased group. The results confirm that determining ADA serum and its isoenzymes, together with CK, LDH and AST values seems to be useful in diagnosing white muscle disease.
2

Retinol, alpha-tocopherol and vitamin D3 in white muscle disease

100%
Mert H., Yildirim S., Yoruk I. H., Irak K., Comba B., Mert I., Aysin N., Comba A.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 07
Vitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fatsoluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions – like eyesight – and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White Muscle Disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, α-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum α-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p < 0.001) and α-tocopherol (p < 0.001) level differences were statistically relevant. Macroscopically, Zenker’s necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker’s necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of α-tocopherol and retinol (p < 0.001) compared to the sick lambs.
3

Studies on serum alpha-tocopherol, selenium levels and catalase activities in lambs with white muscle disease

100%
Kozat S., Gunduz H., Deger Y., Mert N., Yoruk I. H., Sel T.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
This study was conducted to evaluate the effects of combined deficiencies of Se and vitamin E on catalase activity in order to investigate the possible interactions between Se, α- tocopherol, and catalase in lambs with white muscle disease (WMD). Ten healthy and fifteen WMD affected lambs of the Akkaraman breed were used. Serum α-tocopherol and Se levels, and catalase activity were analysed. The catalase activity as well as α-tocopherol and Se levels in lambs with WMD before the treatment (day 0), were found to be lower than those of the control group (P<0.001). Therefore, the catalase activity should be taken into consideration in lambs with WMD.
4

Serum selenium concentrations in water buffaloes (Bubalus bubalis) and black-and-white German Holstein-Friesian cows raised in the Notec River region of the Wielkopolska province

80%
Sablik P., Kobak P., Pilarczyk B., Szarkowski K., Syczewski A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Concentration of selenium in serum samples was determined using a modified Watkinson's spectrofluorometric method. Selenium concentration averaged 0.021 ±0.008 µg/mL for water buffaloes and 0.074 ±0.017 µg/mL for dairy cows. The analysis of selenium concentrations in cows showed deficiency level in 2.0%, marginal level in 64%, and optimal level in 34% of the examined samples. In water buffaloes, deficiency level was found in 96.55% and marginal level in 3.45% of the animals. Selenium levels in cattle and water buffaloes should be periodically diagnosed to provide data for veterinarians to make appropriate decisions. The implementation of suitable prophylactic programmes will enable optimum levels of this element to be maintained.
5

Levels of selected biochemical indicators of serum and blood during subclinical forms of nutritional muscular dystrophy in lambs

80%
Sobiech P., Kuleta Z.
Polish Journal of Veterinary Sciences
|
1999
|
tom 02
|
nr 1
Selenium level, and Ihe activity of GSH-Px, AST, CK, LDH and its isoenzymes were estimated in 30 lambs suspected of having the subclinical form of nutritional muscular dystrophy (NMD) and in 30 healthy lambs. A significant Se deficiency, a reduction in the activity of peroxidase glutathione, an increase in creatine kinase, aspartate aminotransferase and lactate dehydrogenase activities were found in the unhealthy lambs. Isoenzymatic profile of LDH showed 5 tractions. Tń the unhealthy lambs, there was a significant increase in LDH5 fraction-muscular isoenzyme, which is specific to the skeletal muscle. These indicators however, remained within a normal physiological range in the healthy lambs.
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