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A method to estimate the abundance of the fossorial form of the water vole Arvicola terrestris scherman (Shaw, 1801) has been developed, by using surface indices. Results are compared to the standard method of estimation using trap lines. These results show quantitatively that it is possible to differentiate reliably mole indices from water vole indices. Moreover, the two species are inclined to exclude each other. Even though water voles share the same galleries as moles, specific surface indices of the water vole occur for any density exceeding 2 ind/trap line (over 20 ind/ha). Several models of abundance estimation are put forward, all of them using linear multiple regressions. Correlations between the estimations from indices and the estimations from trap lines exceed 0.8 and the limits of using abundance classes are tested. Other limits are developed in the discussion. One of them is that the sampling intervals are saturated for densities exceeding 400 ind/ha. The index method, which is easy to carry out, offers the definite advantage of being suitable to space and time scales otherwise incompatible with estimations from trap lines. For instance, it allows distribution maps from wide transects about areas of more than 25 km2 to be drawn, in less than two days.
In the Early Pleistocene Red Lower Unit of the Sima del Elefante site (Sierra de Atapuerca karst complex, Burgos, Spain), levels TE9–TE13, dental and mandibular remains of an arvicoline are referred to as the new species Arvicola jacobaeus sp. nov. The new species has medium−sized hypselodont molars, with abundant cementum in the re−entrant folds, and thick enamel band with differentiation of the Mimomys−type. The occlusal morphology of M3 is simple. The dental morphology of the new species resembles that of Arvicola sapidus, though smaller. It is more derived, in size and morphology than the Middle Pleistocene species Arvicola mosbachensis. The morphologic affinities among Arvicola jacobaeus, Arvicola terrestris, and A. sapidus suggest a common ancestry. A preliminary phylogenetic analysis corroborates that Mimomys savini is the sister group of the Arvicola clade.
Development of sexual maturity and reproductive activity in Arvicola sapidus Miller, 1908, was studied. The sample analyzed consisted of 374 specimens (208 males and 166 females), captured in various localities in southern Navarre (Spain). Maturity and sexual activity of the males were evaluated both from the cytological analysis of testicular and epididymal tissues and by the testicular and vesicular size. Maturity and sexual activity of the females were determined as a function of the vulva condition, size and vascularization of the uterus, presence of embryos and placental scars, histological analysis of the ovary and development of the mammary glands. The results show that sexual maturity development is related to age, season and size of both the individual and its reproductive organs. In the studied population there are males with spermatozoa present throughout the entire year. However, the size of testes and seminal vesicles of the adult males varied yearly, reaching lowest values at the end of autumn and winter. The mean number of embryos per litter is 3.7 ± 1.4 (ft = 51, range; 1-7), being closely related to the mother's weight. Intrauterine mortality affects at leats 5.1% of the embryos and 17.6% of the litters, while 53.7% of the females show placental scars. Females show high sexual activity between March and October (46.1-90.0% are pregnant or nursing) and are less active in the remaining months. In southern Navarre, A. sapidus breeds throughout the year with two well differen­tiated periods, one of high reproductive intensity (March-October, with maximum between April and June), and another of lesser intensity (November-February).
We tested the influence of nest concealment (vegetation type, density and height), water depth and nest distance from the bank on predation rates upon simulated nests of the water rail (Rallus aquaticus) and the little crake (Porzana parva). Broods of both rallids were simulated by real and wax-filled quail (Coturnix coturnix) eggs coloured typically for each species. Three grades of nest concealment were used: uncovered nests located on wooden floating boards, nests hidden in littoral vegetation and nests hidden under a plastic mesh covered with plants, which made them invisible from the air. Concealment proved to have a stronger impact on the fate of artificial nests than water depth; 95% of the nests on boards were depredated after one week of exposure. Nests attached to a peg at water level and hidden in vegetation had survival rates of 18 and 22%, after three weeks of exposure in two experiment repetitions. In 2012, we found significant differences between survival rates of nests located in different types of vegetation after the first week of the experiment: nests in bulrush (Typha spp.) had a higher survival rate than nests in sedge (Carex spp.) and common reed (Phragmites australis), and nests in sedge had a better survival rate than nests in reed. Those differences disappeared after the end of the experiment. In 2013, nests located in sedge (Carex spp.) had a better survival rate than nests located in bulrush (Typha spp.) or the common reed (Phragmites australis). Covering nests with plastic mesh and plants increased nest survival up to 38% after a three-week-long exposure period. Potential nest predators were monitored: mammals (mustelids and rodents) using live traps and birds by observation of their hunting activity. Filling quail eggshells with wax allowed us to identify the marsh harrier (Circus aeruginosus) as the main nest predator — in 67% of depredated nests, wax eggs carried marks of a raptor beak. American mink (Neovison vison) and the water vole (Arvicola amhibius), though abundant in the study area, were not important nest predators, as only a few bite marks of these mammals were recorded.
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