Operating conditions of the sectors of transport biofuels in Poland are dynamically variable. According to the point of view of some manufacturers, rate of change is too high, especially in the area of legal requirements. We can observe negative impact on the value of the sector and therefore, we can notice that investors’ interest in the sectors of transport biofuels there is on the lower level. The results of the study are not encouraging to invest in the sectors of transport biofuels 1st Generation, slightly better situation there is in terms of 2nd Generation transport biofuels.