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Genetic identification of virulence markers in shiga toxin-producing Escherichia coli [Stec] strains

100%
Osek J.
Bulletin of the Veterinary Institute in Puławy
|
2001
|
tom 45
|
nr 1
E. coli strains isolated from cattle (7 strains) and from children with diarrhea (7 strains) were tested for shiga toxin (stxl and stx2), intimin (eae), and enterohemolysin (ehly) virulence marker genes using PCR. Moreover, the eae genes were further classified by determination of α, β, δ, and ε variants. It was shown that all strains possessed the eae and ehly genetic determinants, as well as genes for stxl (3 strains), stx2 (7 strains) or both toxins (4 strains). PCR amplifications performed with eae-specific primers showed that all 12 O157 isolates were eae γ-positive whereas two strains of O26 serogroup harboured the eae β gene. The obtained results demonstrated that shiga toxin-producing (STEC) E. coli of human and cattle origin isolated in Poland possessed very similar or identical virulence markers.
2

Identification of virulence genes in Campylobacter jejuni and C. coli isolates by PCR

100%
Wieczorek K., Osek J.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 2
Using the PCR technique, the presence of the seven genotypie virulence markers of Campylobacter has been tested. It was found that all C. coli (92 strains) and C. jejuni (105 strains) possessed the flaA and cadF markers. Several strains were positive for the CDT toxin genes (76.6%, 85.3%, and 83.2% strains with the A, B, and C cdt gene variants, respectively). On the other hand, only 66.0% and 20.8% isolates had the iam and virB genes.
3

Relationship between the molecular typing of Campylobacter strains and the prevalence of their virulence genes

84%
Wieczorek K.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 2
193-198
The aim of this study was to evaluate and compare two genotyping methods for the examination of genetic diversity in C. jejuni and C. coli strains isolated from poultry. Moreover, the molecular identification of putative virulence factors was additionally included to establish a correlation between the isolates. Both typing methods, RAPD-PCR and ERIC-PCR, were found to have a high discriminatory power, D=0.95 and 0.94, respectively. The isolates were clustered to 28 groups by RAPD-PCR and to 29 by ERIC- PCR. Sometimes the ERIC or RAPD assays were able to further discriminate the isolates, which had the same virulence profile generated by the second technique. These PCR-based differentiation methods demonstrated the potential for initial rapid epidemiology studies, and when used in conjunction with distribution of virulence markers provide a high resolution strategy for accurately defining subtypes of Campylobacter.
4

Antimicrobial resistance and virulence markers of Campylobacter jejuni and Campylobacter coli isolated from retail poultry meat in Poland

84%
Wieczorek K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The occurrence of Campylobacter in poultry meat and subsequent antibiotic resistance profiles of the isolates were investigated. The prevalence of Campylobacter in 101 samples was 87.1%. Of these samples, 54.5% contained Campylobacter coli and 45.5% were contaminated with Campylobacter jejuni. Among the strains tested, resistance to ciprofloxacin and nalidixid acid was the most common, followed by tetracycline and streptomycin. On the other hand, all analysed isolates were susceptible to erythromycin and gentamycin. Moreover, the prevalence of several virulence marker genes among Campylobacter isolates was estimated. All strains showed the presence of the flaA and cadF factors, whereas the iam was identified only in C. jejuni, while the cdtA, cdtB, and cdtC genes were amplified almost in all C. coli isolates.
5

Identification of the subtilase cytotoxin gene among shigatoxigenic Escherichia coli isolated from different sources

67%
Osek J.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 1
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