Eight ten-week old calves, seronegative to BHVl,were divided at random into 2 groups of four. One group was infected with the Cooper (IBR) strain, while the other group was treated with the 509/89 (IPV) strain. Their humoral immunologic response to the virus was cross tested using the seroneutralisation (SN) test (with both strains) and an immunoenzymatic (IBR - EIA, Svanova) test. However, allergic skin tests using two preparations that were obtained from the above mentioned strains,were applied in the same way as in a tuberculin test. The tests showed that immunogenic properties of the used strains are similar (the mean titres of antibodies in both groups did not differ significantly) but they differ as far as antigenic features are concerned. Lower titres of antibodies were detected with the 508/89 (IPV) strain than with the Cooper (IBR) strain. Humoral immunologic response, tested with EIA, was positive in calves infected with the (IPV) strain (on the 35th day p.i.) and negative in the Cooper (IBR) strain infected group. Allergic tests using „homo- and heterological" preparations, showed that a delayed type of hypersensitivity (DTH) of skin developed in animals from the IPV group on day 35 p.i. (heterological preparation), whereas on day 56 p.i. in the case of the homological preparation. The DTH of skin was maintained up to the end of the experiment. Calves from the IBR group, tested for skin allergy with both preparations, reacted only sporadically positively. The possible reasons for the confirmed differences have been discussed.
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