Wyniki wyszukiwania

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IFNγ production profile in turkeys of different immunological status after TRT vaccination

100%

Smialek M., Tykalowski B., Dziewulska D., Kowalczyk J., Koncicki A.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

2

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The perspective of immunoprophylaxis and selected immunological issues in the course of the turkey rhinotracheitis

72%

Smialek M., Tykalowski B., Stenzel T., Koncicki A.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 1

This review article presents immunological issues in the course of the turkey rhinotracheitis (TRT) emphasizing local immunity mechanisms, both humoral and cell-mediated, in the upper respiratory system. Studies on the influence of the humoral immunity in the course of infection and vaccinations against TRT have revealed many times the absence of correlation between the titre of specific IgY anti-aMPV (avian Metapneumovirus) antibodies in the serum and in the upper respiratory washings and the immunity against the occurrence of the clinical form of the TRT. Considering the above, T cells are increasingly often regarded as the main factor involved in the upper respiratory immunity against the TRT. However, there have been just a few reports on the role of the T cells in the local immunity processes in the infection with aMPV in turkeys. Additionally, studies of the T-cell-associated immunity against the TRT have given ambiguous results. Immunoprophylaxis issues against the aMPV infections are a significant part of the work where the authors confront current vaccination programmes against the perspectives of use of the future vaccines against the TRT. Future vaccines should face the following criteria: absence of the risk of immunosuppressive effect and reversion of vaccine strains virulence, ease-of-use combined with the possibility of administration of the vaccine to the large numbers of turkeys. The leading role in future vaccination programs for birds against the TRT is likely to be played by the in ovo technique and the recombinant vaccines. Great hopes are also linked with the development of subunit vaccines against the aMPV.

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Production of monoclonal antibodies specific to avian pneumoviruses

72%

Bartnicka B., Minta Z.

Bulletin of the Veterinary Institute in Puławy

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1997

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tom 41

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nr 2

Monoclonal antibodies (MAbs) specific to avian pneumoviruses were obtained for the diagnosis of turkey rhinotracheitis (TRT) and swollen head syndrome (SHS). The vaccinal BUT strain of TRT virus was used as an antigen for mouse immunisation. The virus was propagated in a Vero cell culture followed by purification and concentration by ultracentrifugation. The obtained antigen was used for threefold Balb/c mouse immunisation. The spleen from a mouse with the highest anti-TRT antibody level was sampled. Fusion of splenic cells with Sp2/0-Ag 14 myeloma cells was conducted in the presence of PEG-1450. The ELISA and peroxidase-linked assay (PLA) were used to detect antibodies specific to TRT virus in the sera of immunised mice, in the supernatant of hybridoma cell cultures and in the ascitic fluid of mice. The antigenic specificity of MAbs was determined by the immunoblotting technique and their isotype by agar gel immunodifusion method and ELISA. Seven secretive clones were obtained from 2 fusions. The MAbs produced by the clones were detected by the PLA and in the case of 3 clones the ELISA was used. One clone secreted the IgG2a and the remaining clones the lgG1 immunoglobulines. All MAbs recognised the surface G glycoprotein. The MAbs obtained were successfully used to distinguish the Polish avian pneumovirus APV isolates and to detect the virus in the tissues of the respiratory system of chickens infected experimentally.

4

Serological surveillance of avian pneumovirus antibodies in chicken and turkey flocks in Poland

72%

Minta Z., Bartnicka B., Bugajak P.

Bulletin of the Veterinary Institute in Puławy

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1995

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tom 39

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nr 2

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

1 Journal of Veterinary Research

1 Polish Journal of Veterinary Sciences

2 Bartnicka B.

2 Koncicki A.

2 Minta Z.

2 Smialek M.

2 Tykalowski B.

1 Bugajak P.

1 Dziewulska D.

1 Kowalczyk J.

1 Stenzel T.

1 2020

1 2012

1 1997

1 1995

IFNγ production profile in turkeys of different immunological status after TRT vaccination

The perspective of immunoprophylaxis and selected immunological issues in the course of the turkey rhinotracheitis

Production of monoclonal antibodies specific to avian pneumoviruses

Serological surveillance of avian pneumovirus antibodies in chicken and turkey flocks in Poland