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Comparison of cellular and tissue transcriptional profiles in canine mammary tumor

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Pawlowski K. M., Krol M., Majewska A., Badowska-Kozakiewicz A., Mol J. A., Malicka E., Motyl T.

Journal of Physiology and Pharmacology. Supplement

2009

tom 60

nr 1

85-94

Tumor-derived cell lines are widely used as in vitro cancer models. Cell lines historically served as the primary experimental model systems for exploration of tumor cell biology and pharmacology. However, their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line-specific cytogenetic changes. Sometimes cell line studies are interpreted in the context of artifacts introduced by selection and establishment of cell lines in vitro. This complication has led to difficulties in the extrapolation of biology observed in cell lines to tumor biology in vivo. The aim of our study was to compare gene expression profiles in canine mammary tumor tissue and cell cultures derived from those tumors using cDNA microarrays. Tumors of two different origins were used; chondrosarcoma and adenocarcinoma and their primary cell cultures. It has been found that cell culture gene expression profiles closely resembled those of their corresponding in vivo tumor. In adenocarcinoma and chondrosarcoma only 6.0% and 2.7% of genes respectively, have shown significant difference in expression. In the most cases the difference concerned up-regulation of gene expression in cell lines, particularly genes involved in: protein metabolism and modification, signal transduction and nucleotide, nucleoside and nucleic acid metabolism. These experiments revealed that transcriptome of our primary cell culture corresponds to transcriptome of its parental tumor tissue and for this reason cell culture represents the reliable in vitro model for oncogenomic and pharmacogenomic studies.

Ograniczanie wyników

1 Journal of Physiology and Pharmacology. Supplement

1 Badowska-Kozakiewicz A.

1 Krol M.

1 Majewska A.

1 Malicka E.

1 Mol J. A.

1 Motyl T.

1 Pawlowski K. M.

1 2009

Wyniki wyszukiwania

Comparison of cellular and tissue transcriptional profiles in canine mammary tumor