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Effect of recombinant Aea-TMOF on Heliothis virescens

100%

Borovsky D., Iannotti D. A., Shatters R.G.,Jr., Powell C. A.

Pestycydy

|

2005

|

nr 3

79-85

2

Control of mosquito larval trypsin with Aea-trypsin modulating oostatic factor [TMOF] and its analogues

67%

Borovsky D.

Pestycydy

|

2005

|

nr 3

71-77

3

Biological and biochemical effects of organo-synthetic analogues of trypsin modulating oostatic factor [TMOF] on Aedes aegypti, Heliothis virescens and Plutella xylostella

67%

Borovsky D., Nauen R.

Pestycydy

|

2007

|

nr 3-4

17-26

The three-dimensional conformation of Aea-TMOF (Aedes aegypti Trypsin Modulating Oostatic Factor), a decapeptide (YDPAPPPPPP) isolated from mosquito ovaries that inhibits the translation of many trypsin-like serine proteases, e.g. in mosquitoes, flies and lepidopterans, was used as a model for the synthesis of 10 aromatic and aliphatic organic acid and ester analogues. The organic TMOF analogues were tested against herbivorous pest lepidopterans and larval Ae. aegypti. The compounds administered to microtiter plates or to leaf disks caused mortality to mosquito larvae and the diamondback moth, Plutella xylostella, larvae 3-6 days after treatment. The surviving diamondback moth larvae were sluggish, immobile and stopped feeding. A biochemical analysis showed that in larval P. xylostella the trypsin activity was low and correlated with the observed mortality. No activity was found against Heliothis virescens when four of the synthetic compounds that affected P. xylostella and Ae. aegypti were tested, although the decapeptide, TMOF, does affect trypsin biosynthesis in H. virescens.

4

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Control of Aedes aegypti larvae with Aea-TMOF mimics: aromatic derivatives of enoic acid

67%

Borovsky D.

Pestycydy

|

2008

|

nr 1-2

27-34

Based on the 3D conformation of the N-terminus of TMOF in solution, six aromatic derivatives of enoic acid: 7-biphenyl-4-yl-hept-4-enoic acid (BPHE), 7-(4-butyl-phenyl)-hept-4-enoic acid (BuPHE), 7-cyclohexyl-hept-4-enoic acid (CyHE), 10-phenyl-dec-7-enoic acid (PDE), 7-p-tolyl-hept-2-enoic acid ethyl ester (THEEE) and 7-(4-methoxy-phenyl)-hept-2-enoic acid ethyl ester (MPEEE) were computer-modeled and synthesized. Treating first instar Aedes aegypti larvae with different concentrations of the TMOF mimics showed that addition of butyl to the benzyl ring, use of p-tolyl or converting the benzyl ring into cyclohexane increased the biological activity of the mimics by 5.2, 5.0 and 3.8-fold, respectively. Esterifying the carboxyl terminus into ethyl ester and addition of a methoxy group to the benzyl ring also increased, by 2-fold, the biological activity of the derivative. The position of the double bond in the aliphatic chain is important for enhanced biological activity. Aea-TMOF and CyHE fed to mosquito larvae equally inhibited trypsin biosynthesis in larvae for the first 24 h. The biological activity of CyHE, however, rapidly declined 2-3 days later, whereas TMOF activity stayed stable. These results indicate that TMOF organic mimics, although potent, need to be formulated in order to be more stable for future field applications.

Ograniczanie wyników

4 Pestycydy

4 Borovsky D.

1 Iannotti D. A.

1 Nauen R.

1 Powell C. A.

1 Shatters R.G.,Jr.

1 2008

1 2007

2 2005

Effect of recombinant Aea-TMOF on Heliothis virescens

Control of mosquito larval trypsin with Aea-trypsin modulating oostatic factor [TMOF] and its analogues

Biological and biochemical effects of organo-synthetic analogues of trypsin modulating oostatic factor [TMOF] on Aedes aegypti, Heliothis virescens and Plutella xylostella

Control of Aedes aegypti larvae with Aea-TMOF mimics: aromatic derivatives of enoic acid