The aim of the study was to determine the fate in the digestive tract of recombinant CrylA (b) and epsps genes, from genetically modified (GM) insect resistant Bt corn and from glyphosate tolerant GM soybean meal (Roundup Ready). The possibility of the transfer of transgenic DNA from feed to chicken tissues was also evaluated. In a 42-d floor pen experiment, Ross 308 broilers were fed corn-soybean meal diets (55%-60% of corn and 32%-37% of soybean meal). All the experimental diets were isonitrogenous and isoenergetic and contained non-modified corn and soybean meal (group I - control), non-modified corn and GM soybean (group II), GM corn and non-modified soybean meal (group III) or GM corn and GM soybean meal (group IV). At the 43 d of age, the broilers were slaughtered and DNA was extracted from gut content and tissues and was analysed for the presence of transgenic fragments using PCR method. The used methods allowed detecting 0.1% GM DNA in total DNA isolated from samples. The transgenic sequences from single-copy genes of soybean (172 bp) and corn (170 bp) were detected only in content of crop and gizzard of broilers fed GM plants. There were no traces of transgenic DNA in duodenum, jejunum, ileum, and caecum digesta, excreta, and in blood, liver, spleen, and breast muscle. Similarly, no small fragments from other single-copy genes of soybean and corn (recombinant 35s promoter and NOS terminator, and endogenous lectin and invertase genes) were detected in broiler tissues. The obtained data indicated that transgenic DNA sequences from Bt corn and Roundup Ready soybean are well digested in the gastrointestinal tract and are not transferred to broiler tissues.
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