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Agrobacterium-mediated transformation of Bangladeshi Indica rices

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Al-Forkan M., Power J. B., Anthony P., Lowe K. C., Davey M. R.

Cellular and Molecular Biology Letters

2004

tom 09

nr 2

Morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the Bangladeshi Indica rice cultivars BR26 and Binni with Agrobacterium tumefaciens strain LBA4404 carrying the super binary vector pTOK233. Acetosyringone (100 μM) in the medium during coculture (25-28°C) and selection on hygromycin B (50 mg 1-1) were essential for efficient transformation. Stable integration and expression of β-glucuronidase, neomycin phosphotransferase and hygromycin phosphotransferase genes in regenerated plants were confirmed by histochemical and fluorometric assays, ELISA and Southern analysis. Two to 3 copies of T-DNA were integrated into regenerated plants; transgene expression did not correlate with gene copy number. Mendelian segregation of transgenes occurred in T1 seed progeny.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Al-Forkan M.

1 Anthony P.

1 Davey M. R.

1 Lowe K. C.

1 Power J. B.

1 2004

Wyniki wyszukiwania

Agrobacterium-mediated transformation of Bangladeshi Indica rices