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1

Nucleus pulposus repair with cultured autologous elastic cartilage derived chondrocytes

100%
Gorensek M., Joksimovic C., Kregar-Velikonja N., Gorensek M., Knezevic M., Jeras M., Pavlovcic V., Cor A.
Cellular and Molecular Biology Letters
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2004
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tom 09
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nr 2
Low back pain is one of the most common medical conditions in the Western world. Disc degeneration, an inevitable process of ageing, is one of the major causes of low back pain. Autologous chondrocyte transplantation (ACT) is an increasingly popular method of addressing pathological disorders of cartilage. The purpose of our study was to determine whether autologous chondrocytes from elastic cartilage could survive and synthesise a cartilage specific matrix in the intervertebral disc of rabbits. Sixteen lumbar intervertebral discs (IVD) of New Zealand White rabbits were analysed. In 6 IVD, the nucleus pulposus was evacuated and replaced with tissue engineered autologous chondrocytes from auricular cartilage. In the second group, only the nucleus pulposus was evacuated from 6 IVD, with no chondrocytes implantation. Four non-operated IVD were used as a control. Six months after the operation, the animals were euthanized and the IVD were analysed histologically. Autologous cartilage implants were well tolerated by the host for up to six months in vivo. There was only hyaline-like cartilage in the place of the nucleus pulposus. We could not detect any elastic fibres in the new cartilage matrix. In IVD from which only the nucleus pulposus was evacuated and no chondrocytes were implanted, just fibrous tissue was found instead of nucleus pulposus. The overall histological analysis of new cartilage produced after implantation in our study confirmed the hypothesis that ACT from auricular cartilage can be implanted into the IVD instead of the nucleus pulposus and that a significant percentage of implanted chondrocytes survive and produce hyaline-like cartilage.
2

Do mesenchymal stem cells modulate the milieu of reconstructed bladder wall?

84%
Pokrywczynska M., Jundzill A., Bodnar M., Adamowicz J., Tworkiewicz J., Szylberg L., Debski R., Marszalek A., Drewa T.
Archivum Immunologiae et Therapiae Experimentalis
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2013
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tom 61
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nr 6
3

Cryopreserved tissue engineered mucosa

84%
Spoerl G., Mai R., Klingner E., Gedrange T., Lauer G.
Folia Histochemica et Cytobiologica
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2008
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tom 46
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nr 4
541-544
4

Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

84%
Zuber A., Borowczyk J., Zimolag E., Krok M., Madeja Z., Pamula E., Drukala J.
Cellular and Molecular Biology Letters
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2014
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tom 19
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nr 2
Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.
5
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Application of stem cells in dentistry for bone regeneration

84%
Brozek R., Kurpisz M., Koczorowski R.
Journal of Physiology and Pharmacology
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2018
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tom 69
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nr 1
6

Hepatic differentiation from human mesenchymal stem cells on a novel nanofiber scaffold

84%
Ghaedi M., Soleimani M., Shabani I., Duan Y., Lotfi A. S.
Cellular and Molecular Biology Letters
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2012
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tom 17
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nr 1
The emerging fields of tissue engineering and biomaterials have begun to provide potential treatment options for liver failure. The goal of the present study is to investigate the ability of a poly L-lactic acid (PLLA) nanofiber scaffold to support and enhance hepatic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs). A scaffold composed of poly L-lactic acid and collagen was fabricated by the electrospinning technique. After characterizing isolated hMSCs, they were seeded onto PLLA nanofiber scaffolds and induced to differentiate into a hepatocyte lineage. The mRNA levels and protein expression of several important hepatic genes were determined using RT-PCR, immunocytochemistry and ELISA. Flow cytometry revealed that the isolated bone marrow-derived stem cells were positive for hMSC-specific markers CD73, CD44, CD105 and CD166 and negative for hematopoietic markers CD34 and CD45. The differentiation of these stem cells into adipocytes and osteoblasts demonstrated their multipotency. Scanning electron microscopy showed adherence of cells in the nanofiber scaffold during differentiation towards hepatocytes. Our results showed that expression levels of liver-specific markers such as albumin, α-fetoprotein, and cytokeratins 8 and 18 were higher in differentiated cells on the nanofibers than when cultured on plates. Importantly, liver functioning serum proteins, albumin and α-1 antitrypsin were secreted into the culture medium at higher levels by the differentiated cells on the nanofibers than on the plates, demonstrating that our nanofibrous scaffolds promoted and enhanced hepatic differentiation under our culture conditions. Our results show that the engineered PLLA nanofibrous scaffold is a conducive matrix for the differentiation of MSCs into functional hepatocyte-like cells. This represents the first step for the use of this nanofibrous scaffold for culture and differentiation of stem cells that may be employed for tissue engineering and cell-based therapy applications.
7

In-vitro analysis of the expression of TGFbeta-superfamily-members during chondrogenic differentiation of mesenchymal stem cells and chondrocytes during differentiation in cell culture

84%
Goessler U. R., Bugert P., Bieback K., Deml M., Sadick H., Hormann K., Riedel F.
Cellular and Molecular Biology Letters
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2005
|
tom 10
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nr 2
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