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Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers

100%

Sachadyn P., Sobiewska G., Kur J.

Acta Biochimica Polonica

1998

tom 45

nr 3

The proposed here PCR thermal profile improves the specificity and efficiency of PCR using highly degenerate primers, especially in the case of larger PCR products (around 2000 bp and more). The improvement is achieved by the use of a specific annealing temperature in the beginning cycles and the alternate lowering and raising of the annealing temperature in the subsequent cycles.

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Changes in the chemical properties of water in a small pond in vertical profiles

72%

Szyperek U.

Journal of Elementology

2007

tom 12

nr 4

363-373

The study to determine thermal-oxygen changes and chemical properties of water in a flow-through pond was conducted between April 2004 and March 2005 in three vertical profiles. The study involved the determination of electrolytic conductivity and pH reaction of water as well as the content of nitrate nitrogen and ammonium nitrogen, total phosphorus and sulphates. Thermal stratification was not observed in the investigated pond with the maximum depth of 2.45 m, and oxygen content was represented by a clinograde curve. Biogenic element concentrations in the pond were determined by depth in the vertical profile, and they were subject to seasonal variation. The highest concentrations of mineral substances in water were reported in the bottom layers of the pond. Although the investigated pond is a flow-through water body, it accumulates biogenic elements.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Elementology

1 Kur J.

1 Sachadyn P.

1 Sobiewska G.

1 Szyperek U.

1 2007

1 1998

Wyniki wyszukiwania

Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers

Changes in the chemical properties of water in a small pond in vertical profiles