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Succinate dehydrogenase and acid phosphatase activity in Phaseolus lunatus testa

100%

Aniszewski T.

Acta Biologica Cracoviensia. Series Botanica

2006

tom 48

nr 2

Succinate dehydrogenase (SDH) and acid phosphatase (AcP) activity in Phaseolus lunatus seed testa are demonstrated in enzyme tests, and uptake and transport of vital and indicator dyes such as methylene blue, Congo red and tetrazolium in the seed testa are examined by light and transmission electron microscopy. SDH activity was observed in the vascular bundles (endotesta) and in some cells in endo- and mesotesta. AcP activity was located near cell walls in both meso- and endotesta. In the vascular bundles there was very little AcP activity. Vital and indicator dyes were conducted from the exotesta (hilum) to endotesta. Vesicle mobilization was observed in the mesotesta. Strong enzyme activity in the meso- and endotesta and vesicle mobilization in the mesotesta suggest the potential active role of testa strata in imbibition and the initial nutritional stage of germination.

Structure of Phaseolus lunatus testa at its central point

100%

Aniszewski T., Karttunen A. L., Hyvarinen H.

Acta Biologica Cracoviensia. Series Botanica

2006

tom 48

nr 1

In the Phaseolus lunatus seed, the strata and cells of the testa at the central point of the vertical side are described. The proposed regionalization and the central point technique makes it possible to define precisely each site in the testa. Exo-, meso- and endotesta are found to be basic strata in the central point studied. The exotesta is divided into cuticle, palisade and crushed cell layers. The cuticle layer is irregular and noncellular. The palisade cells are exoepidermal sclerenchyma and the crushed cells are former exoepidermal cells. The mesotesta is composed of hourglass cells, called stapes cells here because their form resembles stapes, and crushed cells. The stapes cells are parenchyma. The endotesta is composed of support tissue, three crushed cell layers, phloem, xylem and testa bottom. The endotesta adjoins the cotyledon.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

2 Aniszewski T.

1 Hyvarinen H.

1 Karttunen A. L.

2 2006

Wyniki wyszukiwania

Succinate dehydrogenase and acid phosphatase activity in Phaseolus lunatus testa

Structure of Phaseolus lunatus testa at its central point