Wyniki wyszukiwania

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Zastosowanie testu transformacji blastycznej limfocytów w immunologii weterynaryjnej

100%

Szeleszczuk P., Zaleska M., Szeleszczuk M.

Życie Weterynaryjne

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1989

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tom 64

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nr 07

2

Test transformacji limfocytów (LTT)

88%

Rychlik U., Marszalek A., Sygitowicz G.

Diagnostyka Laboratoryjna

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2013

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tom 49

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nr 4

3

Test transformacji blastycznej limfocytów w ocenie wpływu ochratoksyny A na układ odpornościowy kur i ich potomstwa

63%

Zaleska M., Zbikowski A., Niemiec J., Kosowska G.

Medycyna Weterynaryjna

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2011

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tom 67

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nr 04

Ochratoxin A (OA) is the most important mycotoxin causing contamination of feed and food. In addition to strong nephrotoxicity, OA also affects the immune system. Using a test of the blastic transformation of lymphocytes (TBTL) the impact of OA on the proliferative capacity of peripheral blood lymphocytes in laying hens and their offspring has been evaluated. In the first experiment the authors evaluated the influence of feeding hens for 14 days on feed containing 0.5 ppm of ochratoxin A. Blood samples were collected at day 14 of the experiment. The studies to determine the effect of ochratoxin on the proliferative capacity of lymphocytes in the offspring of hens fed a diet containing the mycotoxins were performed in three groups of broiler breeders. Birds received a mix containing 0.5 ppm of OA for 4 weeks. The collection of eggs for hatching was started after 7 days of feeding hens with feed contaminated by OA. Two test hatchings were conducted. Hatched chicks were reared under standard conditions. At 14, 21, 35 and 49 days of rearing chickens were bled for testing TBTL. The authors have shown a suppressive effect of ochratoxin A contained in the feed given to hens on the proliferating ability of lymphocytes (mean IS in treatment and control groups were, respectively, 7.30 and 9.41). The study shows that OA in feed given to hens also reduced the stimulation index of peripheral blood lymphocytes in their offspring.

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Techniki uzyteczne w ocenie swoistej odpornosci komorkowej

51%

Pomorska-Mol M., Markowska-Daniel I.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 05

306-309

In the era of the excessive use of chemotherapeutics, especially antibiotics and an increase of bacterial strains resistance, studies concerning the stimulation of the immune system and evaluation of its activity seem to be particularly important. This article presents a review of the methods useful in the evaluation of cellular immunity. The evaluation of specific cell-mediated immunity may be performed in vivo or in vitro. Delayed-type hypersensitivity assay is one that is possible to make in vivo, while the lymphocyte transformation test, cytometric proliferation assay, evaluation of activation marker expression and analysis of antigen-specific production of cytokines by sensitive lymphocytes T are methods performed in vitro. The evaluation of cell-mediated immunity in immunological studies, especially concerning vaccinology, will be valuable or even essential elements of an experiment.

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Diagnostyka Laboratoryjna

1 Życie Weterynaryjne

2 Zaleska M.

1 Kosowska G.

1 Markowska-Daniel I.

1 Marszalek A.

1 Niemiec J.

1 Pomorska-Mol M.

1 Rychlik U.

1 Sygitowicz G.

1 Szeleszczuk M.

1 Szeleszczuk P.

1 Zbikowski A.

1 2013

1 2011

1 2009

1 1989

Zastosowanie testu transformacji blastycznej limfocytów w immunologii weterynaryjnej

Test transformacji limfocytów (LTT)

Test transformacji blastycznej limfocytów w ocenie wpływu ochratoksyny A na układ odpornościowy kur i ich potomstwa

Techniki uzyteczne w ocenie swoistej odpornosci komorkowej