A study group of six horses was immunized twice (day 0 and 29 of the experimental) with an inactivated vaccine (Solvay-Duphar, the Netherlands). The control group was formed of six unvaccinated horses. Blood for hematological examinations, cellular immune responses, specific humoral immunity (HI, ELISA, SRH) was collected on days 0, 14, 29, 43, 63, 131, 138 and 182 of the experiment. A viral challenge of 3 vaccinated and 3 nonvaccinated horses was made on day 131. One of the animals from each of these two groups was challenged intranasally with viral subtype A1 (Prague/56), another with A2 (Miami/63) and the third with A2 (Suffolk/89). It is worth noting that 14 days after first vaccination a statistically significant increase of the lymphocyte stimulation index for the A2 subtype was observed in vaccinated horses. At the same time the percentage of rod neutrophils was also significantly elevated in the same group. This was not found in nonvaccinated horses at the same time, but it was observed in that group of animals after viral challenge. The leukocyte migration inhibition index was significantly higher for the A2 subtype in vaccinated horses during the whole experiment. It was not found for the A1 subtype. A statistically significant increase of the specific antibody titres was observed in vaccinated horses after the second vaccination (HI, ELISA, SRH). It was also determined after the challenge in vaccinated and nonvaccinated horses, but only in the SRH test. A total correlation (regression) between ELISA and SRH tests was observed before the viral challenge. Attempts to reisolate the virus from nasal cavities of the horses were unsuccessful.
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