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Biomass allocation pattern is an important plant characteristic which influences how plants respond to abiotic and biotic heterogeneity. Prior studies indicate that above-ground biomass scales nearly isometrically with respect to below-ground biomass regardless of environment or phyletic affinity. However, such rule has been mostly tested with data on trees and usually without drought stress. Given the importance of this predicted relationship, it should be evaluated for a wider range of species and environmental conditions. Variations of the above- and belowground biomass (MA and MR, respectively) were determined from five sites in north-west China, which compose a natural moisture gradient (aridity index ranging from 0.95 to 1.98). Model Type II regression protocols were used to compare the numerical values of MA vs MR scaling exponents (i.e. slopes of log-log linear relationships). The resulting five scaling exponents were indistinguishable and had a similar, nearly isometric slope (i.e. MA ∞ MR ͌ ¹‧⁰). Significant variation was observed in the Y-intercepts of the five regression curves, because of the absolute differences in MA or MR. These results support prior allometric theory, which reveals an isometric relationship between above- and below-ground biomass, and may provide a suitable method to estimate the regional below-ground biomass based on the direct aboveground measurements.
Eighty four different fungal endophytes isolated from sea grasses (5), marine algae (36) and leaves or barks of forest trees (43) were grown in vitro and the secondary metabolites secreted by them were harvested by immobilizing them on XAD beads. These metabolites were eluted with methanol and screened using SYBR Green I assay for their antiplasmodial activity against blood stage Plasmodium falciparum in human red blood cell culture. Our results revealed that fungal endophytes belonging to diverse genera elaborate antiplasmodial metabolites. A Fusarium sp. (580, IC50: 1.94 μg ml−1) endophytic in a marine alga and a Nigrospora sp. (151, IC50: 2.88 μg ml−1) endophytic in a tree species were subjected to antiplasmodial activity-guided reversed phase high performance liquid chromatography separation. Purification led to potentiation as reflected in IC50 values of 0.12 μg ml-1 and 0.15 μg ml−1 for two of the fractions obtained from 580. Our study adds further credence to the notion that fungal endophytes are a potential storehouse for a variety of novel secondary metabolites vested with different bioactivities including some that can stall the growth of the malaria parasite.
Ecological stoichiometry is the study of the balance of multiple chemical elements in ecological interactions. Terrestrial plants exhibit inter- and intra-specific differences in leaf C:N:P stoichiometry that has been used to study plant competition and succession, nutrient use efficiency, N and P nutrient limitation. However, little is known about leaf C:N ratio, N:P ratio in the Loess Plateau, particularly the pattern of leaf C:N:P stoichiometry during the secondary succession. Thus, leaf stoichiometric traits and specific leaf area (SLA) of dominant species at different secondary successional stages in the Loess Plateau were measured. The study was conducted at the Lianjiabian forest region of the Loess Plateau (35.03°–36.37°N and 108.10°–109.08°E), Gansu, China. Leaf C:N:P stoichiometry and specific leaf area of 18 dominant species (herb) community stage (Stipa bungeana Trin, Bothriochloa ischaemun (Linn.) Keng, Carex lanceolata Boott, Artemisia sacrorum Ledeb, Pulsatilla chinensis (Bunge) Regel, Potentilla chinensis Ser), shrub community stage (Sophora viciifolia Hance, Hippophae rhamnoides (Linn.), Ostryopsis davidiana Decne, Rubus palmatus Thunb, Rosa xanthina Lindl, Acer ginnala Maxim, Spiraea pubescens Turcz, Ziziphus jujuba var. spinosa), early forest community stage (Populus davidiana Dode, Betula platyphylla Suk, Platycladus orientalis (Linn.) Franco), climax forest community stage (Quercus liaotungensis Koidz)) at four secondary successional stages were determined in the Loess Plateau in early June of 2006. The herb and shrub community stage had higher SLA than that in the early forest community stage and climax forest community stage. The Leaf C, N, C:N ratio and N:P ratio at different secondary successional stages were significantly different (P <0.05), but the leaf P didn’t differ. The N:P ratio of herb community stage were significant difference from those of the others stages (shrub community stage, early forest community stage and climax forest community stage), but there were not significant difference among the shrub community stage, early forest community stage and climax forest community stage. N:P ratio shifts of the species were consistent along the successional sere, although the N:P ratio of the different species at a successional stage varied considerably. On community level, the lowest N:P ratio (9.8) was found in herb community stage in the secondary succession, the N:P ratio increased to 12.9 in shrub community stage, declined to 12.4 in early forest community stage, and increased in climax forest community stage (e.g. 12.6 in the Quercus liaotungensis Koidz community). The results suggest that the vegetation productivity of the Loess Plateau was N-limited at each secondary successional stage.
The content of total lipids and lipofuscins was determined in leaves of S. latifolium aerial-aquatic and terrestrial plants at the flowering and fruiting phases. There were no significant differences in the lipid total content between aerial-aquatic and terrestrial plants at the flowering phase. At the fruiting phase, the lipid content was lower in terrestrial plants. Lipofuscins, the so-called senescence pigments, were determined in plants of both ecotypes at the flowering phase, although visible symptoms of senescence were absent. At the fruiting phase, there was an accumulation of lipofuscins in aerial-aquatic and terrestrial plants that may be explained by progressive senescence. The lipofuscin content was higher in terrestrial plants at both phases of ontogenesis. We concluded that earlier and more intensive senescence is typical for terrestrial plants, which are in the conditions of chronic moderate water deficit on the riverside.
It is known that the dominance of graminoid species is promoted by N addition; however, there has been relatively little effort to examine the pattern induced by natural N fertility. Since nutrie nt use e fficiency (NUE) is an important trait determining plant competitive ability, we expected that the species guild with higher NUE (lower nutrient content) may be more competitive on infertile soils. We explo red t he relationships between relative forbs biomass share , soil N and productivity by the linear regressi on analysis on a natural alpine meadow in northeast of Qinghai- Tibetan Plateau (3600 m a.s.l.). To test the variety of leaf N:P stoichiometry, paired t test and general linear model multivariate (GLM) analysis were also used. We f ound that the leaf N:P ratios of the whole community were below 13 in studied sites, which may be consistent with the N limitation on the veget ation. Graminoids re tained lower concentrations of leaf N and P than forbs in community on the Nlimited grassland. Consistent with our prediction, we found that the biomass fraction of graminoids declined with soil N content and aboveground production on the grassland. Different from the pattern along fertility gradients induced by N fertilization, our results showed that gr aminoids with lower internal nutrient content w ere able to resist low levels of nutrient availability on the natural alpine grassland when compared to forbs.
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