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Tanshinones in culture of Salvia przewalskii maxim in vitro

100%
Skala E., Mielicki W., Wysokinska H.
Acta Biologica Cracoviensia. Series Botanica
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2014
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tom 56
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nr 1
2

Influence of development stage on the content of biologically active compounds of Salvia miltiorrhiza Bunge roots

75%
Buchwald W., Mrozikiewicz P. M.
Herba Polonica
|
2007
|
tom 53
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nr 4
Studies on contents of phenolic compounds and diterpenes in Salvia miltiorrhiza Bunge root during the vegetation period were carried out. In Polish climate and soil condition, the tanshinone II A occurred as the dominant compound from diterpenes group. Obtained results show that the most profitable period for harvesting Salvia miltiorrhiza raw material is fructification stage. However, it is very difficult to decide on the accurate harvest date in this period. After all, taking into account various weather conditions, harvest of the Salvia mitiorrhiza Bunge should be done at the end of the vegetation period (in November).
3

The effect of mineral fertilization on the content of biologically active compounds and mass of roots of Salvia miltiorrhiza Bunge

63%
Buchwald W., Mrozikiewicz P. M.
Herba Polonica
|
2007
|
tom 53
|
nr 4
The research of mineral fertilization effect of Salvia miltiorrhiza Bunge has been carried out in 2004–2006. It was found that potassium fertilization had the biggest influence on the amount of dry root mass changeability. The evaluation of mineral fertilizer effect of biological active compounds gave ambiguous results. In the raw material of Polish cultivation origin the phenolic acids and tanshinones, which determine the healing properties of Salvia miltiorrhiza, were noticed. In the roots the tanshinone IIA occurred to be the dominating substance from diterpenes group. The weather conditions significantly influenced the amount of biological active compounds.
4

Salvia miltiorrhiza BUnge in vitro cultivation in callus cultures

63%
Krajewska-Patan A., Dreger M., Gorska-Paukszta M., Mscisz A., Mielcarek S., Baraniak M., Buchwald W., Marecik R., Grajek W., Mrozikiewicz P. M.
Herba Polonica
|
2007
|
tom 53
|
nr 4
Salvia milthiorrhiza root (Danshen) is one of the oldest and most traditional drug of Chinese origin, mainly used in the treatment of cardiovascular and cerebrovascular diseases. The tanshiniones (diterpenoids) and phenolic acids are the main biological active substances of S. miltiorrhiza. The aim of this study was to determine the optimal conditions for callus cultures and biosynthesis of the biological active compounds. The callus cultures (on solid medium, CC A in shake flask and CC A in bioreactor) were obtained and phytochemical studies on them were carried out. Total amount of phenolic acids determined in callus (solid medium) averaged from 2.58% to 5.72% of dry weight (DW). The callus cultured on solid medium and CC A (in flasks) did not produce tanshiniones. Culture conditions in the bioreactor enabled the biosynthesis of tanshiniones (0.27% of dihydrotanshinione, 0.12% of cryptotanshinone, 0.01% of tanshinione 2A and tanshinione 1). The obtained contents of rosmarinic acid in callus on solid medium and CC A (cultured in shake flasks) are relatively high and comparable to raw material. The callus cultured in bioreactor is eligible for tanshinione production, moreover the accumulation of them is comparable with the intact plants.
5

Microbiological study of extracts of Salvia miltiorrhiza Bunge roots

63%
Buchwald W., Kedzia B., Baraniak M.
Herba Polonica
|
2007
|
tom 53
|
nr 4
Roots of Salvia miltiorrhiza Bunge contain mainly fractions of water-soluble substances. Most tanshinones are found in ethyloacetate fractions, slightly less in hexane extract. Tanshinones in water extracts have not been found. Ethyloacetate and hexane extracts from Salvia miltiorrhiza Bunge root are characterized by high microbiological activity (MIC range of 10–75 μg/ml using standard strain Staphylococcus aureus FDA 209P). The highest activity and maximum amount of cryptotanshinone was found in extracts obtained from roots of Polish origin. The microbiological activity of water fraction is quite low regardless of the source of raw material (MIC range 500-1000 μg/ml). The results suggest that the microbiological activity of Salvia miltiorrhiza Bunge extracts depends on the quality and quantity of tanshinones composition.
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