The purpose of this study was to determine differences in activity of aminoacyl-tRNA synthetases and bonding effectiveness of amino acids by tRNA in the livers of rats receiving mercury chloride in their drinking water. The experiments were conducted on Wistar rats divided into two groups. The first group received 100 mg/1 doses of mercury (mercury chloride) in drinking water, the second was a control. After 6 weeks aminoacyl-tRNA synthetases and tRNA were obtained from the rat’ livers. The crude preparates of aminoacyl-tRNA synthetases were obtained by precipitation with ammonium supersulphate in the 40-70% range and tRNA was obtained by phenol extraction. The activity of aminoacyl-tRNA synthetases and bonding effectiveness of amino acids by tRNA were tested in vitro using incubation mixtures. The results showed that the activity of ten tested aminoacyl-tRNA synthetases was lower in livers of rats which received mercury chloride in drinking water than those in the control group. Moreover, the bonding effectiveness of amino acids by tRNA was lower in these rats’ livers.
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