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1

Phylogenetic analysis of selected strains of rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60

100%
Pawlikowska M., Hukowska-Szematowicz B., Deptula W.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 2
129-133
The purpose of the study was the phylogenetic analysis of 16 European strains of the rabbit haemorrhagic disease (RHD) virus identified in 1989-2004, on the basis of N-terminal fragment of the gene encoding structural protein VP60. The obtained sequences of the strains were compared to the sequences of 30 strains of the virus received from the GenBank gene database. As a result of phylogenetic analysis of all the strains, they were divided into six genogroups, which were principally formed on the basis of the time of their identification, and the place of isolation of the RHD virus, which confirms the present hypothesis of genogroups creation among this virus. Furthermore, in our studies, a clear and visible difference was obtained between the classic strains in genogroups 1-5, and the strains referred to as antigen variants - genogroup 6.
2

Cystocyte and lymphocyte derived fusomes-spectrosomes: analogies and differences: a mini-review

100%
Dubielecka P. M., Stebelska K., Jazwiec B., Sikorski A. F.
Cellular and Molecular Biology Letters
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2003
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tom 08
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nr 1
Structures analogous to Drosophila spectrosomes were found in mammalian lymphocytes. Repasky and colleagues discovered an intracellular spectrin-rich structure in lymphoid cells, which had far-reaching parallels with the fusome/spectrosome of D. melanogaster germ cells. This fact implies that spectrosomes may be characteristic not only of insect germ cells, but also that an analogous structure may play an important role in other cell types. The term “spectrosome” was first used by Lin and Spradling in 1995 to describe a large sphere of fusomal material in D. melanogaster germline stem cells and their differentiated daughter cells - cytoblasts. In the D. melanogaster ovary, membrane skeletal proteins such as ankyrin, α/β spectrin as well as adducin-like Hts protein(s) were found in this specific organelle - spectrosome/fusome. These organelles are involved in the creation of mitotic spindles and D. melanogaster cyst formation and oocyte differentiation, but the role of analogous spectrin based aggregates found in nucleated cells still remains unclear.
3
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Rabbit haemorrhagic disease virus: studies on field strains isolated in Poland

100%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
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1996
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tom 40
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nr 2
The standard strain and 78 field samples of rabbit haemorrhagic disease were tested by the haemagglutination test (HA) and ELISA. The occurrence of 17% false negative results in HA test was demonstrated. The results of studies of 30 out of 78 samples examined by the ELISA prepared in our laboratory in comparison with commercial kit are presented. The high correlation of results obtained in ELISA kits was observed. The structural proteins of 17 viral sarnples were analysed by the SDS-Page and Western blot methods. HA positive samples demonstrated the presence of major structural protein with molecular weight of about 60 kD and in some samples the protein with m.w. of 38 kD. In HA false negative samples the proteins of about 60 and 26-28 kD were demonstrated. The viral proteins recognized by positive rabbit homologic and hare heterologic sera, did not react with negative rabbit serum.
4

Phylogenetic analysis of French strains of the RHD virus on the basis of a fragment of gene encoding C-terminal part of structural capsid VP60 protein

80%
Hukowska-Szematowicz B., Deptula W.
Advances in Agricultural Sciences
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2010
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tom 13
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nr 1
5

Characteristics of genes encoding structural protein of Polish strains of goose parvovirus

80%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 2
119-122
The aim of this study was to come up with molecular characteristics of Polish strains of goose parvovirus (GPV) on the basis of genes encoding their structural proteins. Ten field GPV strains and two vaccine strains: B-38 and MFP were used. The PCR- RFLP method based on three distinct endonucleases: HinfI, MboI, and RsaI, which had restriction sites within the sequences of examined genes, was applied. It was found that the homology of VP1, VP2, and VP3 among the studied strains ranged from 50% to 100%. The major differences in restriction patterns were found after application of HinfI, whereas 100% homology was observed after Rsal digestion. Significant differences were observed in restriction profiles of vaccine GPV strains. The study revealed that the application of PCR-RFLP for the analysis of VP1, VP2, and VP3 genes allowed for their molecular characteristics and differentiation between the vaccine and field strains.
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