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The effects of essential (Zn⁺²) and non-essential (Cd⁺² and Pb⁺²) heavy metals on morphogenesis of two represantatives of informal group zoosporic fungi namely; Saprolegnia delica Coker and Dictyuchus carpophorus Zopf. were studied. These two species varied in their tolerance of each amended heavy metal. Lead had the most potent effect amongst the tested heavy metals in inhibiting the radial extension of the vegetative hyphae of the two tested species. The vegetative hyphae of S. delica and D. carpophorus assumed different morphological alterations compared with that at controls depending upon the applied heavy metal and the dose concentration. Both zoosporangial formation and discharges of the two tested fungi were greatly inhibited even at the low concentrations of Cd. Zoosporangia of D. carpophorus appeared curved at high concentrations of Cd. Zoosporangial formation and discharge of the two zoosporic fungi showed variable defonnation when treated with Pb. The different applications of Zn nearly stimulated sporangial elongation in both zoosporic fungi. Sex organs varied in their numbers and morphogenesis at each treatment of the applied heavy metal. The gemmae of S. delica were greatly reduced or missed at the elevated toxic levels of Cd whereas they enhanced in numbers and size at most Pb treatments and little affected at Zn applications.
A correlation is known to exist in yeast and other organisms between the cellular resistance to stress and the life span. The aim of this study was to examine whether stress treatment does affect the generative life span of yeast cells. Both heat shock (38°C, 30 min) and osmotic stress (0.3 M NaCl, 1 h) applied cyclically were found to increase the mean and maximum life span of Saccharomyces cerevisiae. Both effects were more pronounced in superoxide dismutase-deficient yeast strains (up to 50% prolongation of mean life span and up to 30% prolongation of maximum life span) than in their wild-type counterparts. These data point to the importance of the antioxidant barrier in the stress-induced prolongation of yeast life span.
Salinity stress is a serious problem in urban landscape of arid and semi arid regions. To overcome the adverse impact of salinity, the application of organic matter and plant nutrients in the growth media for improving the plant growth is essential. An experiment was conducted in order to determine the response of Salvia splendens to salinity levels and also the role of humic acid in the salt stress alleviation. In the current experiment, five salinity levels (0, 20, 40, 60, and 80 mM NaCl) and three humic acid (0, 100, 500 and 1000 mg/l) treatments were prepared. The effects of these treatments were investigated on some growth parameters, physiological characteristics and also biochemical compounds. The results indicated that the growth parameters decreased in saline-treated than control plants. Different salinity levels significantly affected relative water content, evaporation rate and also electrolyte leakage. Salinity caused the increase in proline, malondialdehyde, sugar content, DPPH, total phenol content and decrease in chlorophyll, compare to the control plants. Application of humic acid on Salvia splendens decreased the leaf area and plant height compared to the control plants. Thus, regarding the growth parameters, it is probable that the effect of humic acid on the biochemical compounds is similar to salinity effect. The findings suggest that sage is an ornamental plant sensitive to salinity and humic acid (in the studied levels) could not alleviate the negative effects of salt stress on this plant.
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