Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Characterization of rinderpest virus vaccine strains by molecular biology techniques

100%

Ayanot P. K., Kolosov S. N., Lomakin A. I., Starov S. K., Drygin V. V., Gusev A. A.

Bulletin of the Veterinary Institute in Puławy

|

1998

|

tom 42

|

nr 1

2

Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing

86%

Zaczek A., Brzostek A., Gorna A., Sajduda A., Wojtasik A., Dziadek J.

Polish Journal of Microbiology

|

2013

|

tom 62

|

nr 1

The current “gold standard” in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discriminatory power of FLiP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative to the other typing methods.

3

Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

72%

Dobrowolska A., Debska J., Kozlowska M., Staczek P.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 2

Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.

4

Molecular analysis [RAPD-PCR] of inter-strain hybrids of the Paramecium aurelia species complex [Ciliophora, Protozoa]

72%

Przybos E., Prajer M., Greczek-Stachura M.

Folia Biologica

|

2005

|

tom 53

|

nr 3-4

Ograniczanie wyników

2 Polish Journal of Microbiology

1 Bulletin of the Veterinary Institute in Puławy

1 Folia Biologica

1 Ayanot P. K.

1 Brzostek A.

1 Debska J.

1 Dobrowolska A.

1 Drygin V. V.

1 Dziadek J.

1 Gorna A.

1 Greczek-Stachura M.

1 Gusev A. A.

1 Kolosov S. N.

1 Kozlowska M.

1 Lomakin A. I.

1 Prajer M.

1 Przybos E.

1 Sajduda A.

1 Staczek P.

1 Starov S. K.

1 Wojtasik A.

1 Zaczek A.

1 2013

1 2011

1 2005

1 1998

Characterization of rinderpest virus vaccine strains by molecular biology techniques

Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing

Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

Molecular analysis [RAPD-PCR] of inter-strain hybrids of the Paramecium aurelia species complex [Ciliophora, Protozoa]