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1

Replication of equine herpesvirus type 1 in equine dermal cells transfected with Bam HI[G] restriction fragment of EHV-2 genome

100%
Dzieciatkowski T., Chmielewska A., Turowska A., Tucholska A., Banbura M. W.
Polish Journal of Veterinary Sciences
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2009
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tom 12
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nr 1
In previous experiments, we have demonstrated that the presence of equine herpesvirus 2 (EHV-2) enhanced plaque formation in cell cultures infected with equine herpesvirus type 1. To determine whether a specific region of the EHV-2 genome is responsible for this effect, we have constructed a library of Bam HI fragments of the EHV-2 genome ligated into pcDNA plasmid. Equine dermal (ED) cell cultures were subsequently transfected with the constructs, passaged 5 times, tested for the presence of the plasmids and infected with EHV-1 at MOI=0.01. Only in cultures transfected with the pcDNA/Bam HI[G]construct, designated Δ2/4, the mean number of plaques at 24 hrs p.i. was approximately 10 times higher than in non-transfected controls. Virus titers in culture supernatants as well as in freeze-thawed cells were 4- and 5-fold higher, respectively, than in non-transfected cultures. These differences were observed only at 24 hrs p.i. At 48 hrs p.i. cultures were completely destroyed and, surprisingly, the virus titer was slightly lower in the supernatant of transfected cells. However, the titer of EHV-1 in freeze-thawed culture was exactly the same as in the control. These results suggest that the presence of Bam HI[G] fragment of the EHV-2 genome stimulates (accelerates?) plaque formation only at earlier stages of infection but does not influence the total yield of EHV-1 at 48 hrs p.i. The exact mechanism of this stimulation remains unclear and further experiments are necessary to determine the role of putative EHV-2 proteins encoded by Bam HI [G] fragment of the EHV-genome.
2

Comparison of stimulatory effect of auxins and cytokinins on protein, saccharides and chlorophylls content in Chlorella pyrenoidosa Chick

84%
Czerpak R., Krotke A., Mical A.
Polskie Archiwum Hydrobiologii
|
1999
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tom 46
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nr 1
3

Stimulatory effect of interleukin-2 on cell proliferation of prolactin secreting pituitary tumors in vitro

84%
Kunert-Radek J., Stepien H.
Folia Histochemica et Cytobiologica. Supplement
|
1997
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tom 35
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nr 2
4

Stimulatory effect of 2,3,5-triiodobenzoic acid (TIBA) on shoot growth and flowering of partially cooled tulip (Tulipa gesneriana L.) bulbs

67%
Saniewski M., Okubo H., Miyamoto K., Oka M., Ueda J.
Journal of Fruit and Ornamental Plant Research
|
2011
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tom 19
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nr 2
In the present work, 2,3,5-triiodobenzoic acid (TIBA) was applied to uncooled tu­lip bulbs, cultivars Apeldoorn and Gudoshnik, before flower bud formation, at the beginning of July and after flower bud formation, in October and November. Shoot growth and flowering of partially dry-cooled bulbs were substantially stimulated. These results strongly suggest that TIBA partially replaces the cold requirement of the tulip bulbs. In addition, the effect of TIBA is similar to gibberellins applied exogen- ously to the bulbs. Such a gibberellin application partially substitutes for cold treat­ment. Gibberellin application stimulates shoot growth and flowering of tulips. The mode of action of TIBA is discussed in relation to auxin action in tulips.
5
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Stimulatory effect of Asahi SL on selected plant species

67%
Przybysz A., Wrochna M., Slowinski A., Gawronska H.
Acta Scientiarum Polonorum. Hortorum Cultus
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2010
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tom 09
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nr 2
Asahi SL stimulates plant’s vital processes like growth and development, affects physiology and biochemistry, what often leads to increased biomass accumulation and yield. However, common is opinion that application of this preparation could be beneficial only, when treated plants are grown under unfavorable conditions. Therefore the aim of this work was the assessment of the stimulatory effect of Asahi SL on Arabidopsis thaliana L. and ornamental amaranth plants grown under optimal conditions. Plants treated with Asahi SL were higher and more advanced in development, particularly generative. Biomass accumulation was greater after biostimulator application mainly due to better photosynthetic apparatus efficiency, which was manifested by (i) greater leaf area, (ii) higher total chlorophyll content and (iii) increased intensity of photosynthesis. Effect of Asahi SL on chlorophyll a fluorescence was marginal. Despite of higher transpiration and lowered stomatal resistance the RWC was almost unchanged in biostimulator treated plants what was attributed to increased water uptake. Obtained results clearly showed that Asahi SL applied on plants can also be effective and beneficial when they are grown under optimal conditions.
6

Effects of auxins on growth and anthraquinone production in Rheum palmatum L. callus cultures

67%
Siatka T., Kasparova M., Sicha J., Dusek J.
Herba Polonica
|
2003
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tom 49
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nr 1-2
Effects of four auxins (2,4-dichlorophenoxyacetic acid, a-naphthaleneacetic acid, ß-indole- acetic acid or ß-indoiebutyric acid) at the concentrations of 0.1, 1 or 10 mg/I on the cell growth and anthraquinone production in Rheum palmatum L. callus cultures were stud­ied. The highest growth was obtained by lmg/l 2,4-D and 10 mg/l NAA (growth index 9.29 and 9.60, respectively), the best anthraquinone production was achieved by 1 mg/1 NAA, 10 mg/1 NAA, 1 mg/l IAA and 0.1 mg/1 IBA (5.24-5.51 mg/g diy weight).
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