Wyniki wyszukiwania

1

Self-injuries in adolescents: social competence, emotional intelligence, and stigmatization

100%

Kulikowska A., Pokorski M.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 6

383-392

2

Proteins of stigma exudate.

100%

Kalinowski A., Radlowski M., Bartkowiak S.

Journal of Applied Genetics

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1996

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tom 37A

3

Tapetal raphides of Tradescantia bracteata: the strategy of their transport from anther to stigma

84%

Lesniewska J., Och B., Charzynska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

4

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Adventitious bud regeneration from the stigma of Sinapis alba L.

84%

Zarychta L., Zenkteler E.

Acta Agrobotanica

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2010

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tom 63

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nr 1

Stigmas isolated from flower buds of ‘Nakielska’ variety of Sinapis alba were used to develop a micropropagation method suitable for breeding of new cultivars. The origin of adventitious bud regeneration was studied on MS medium, under stimulation by bezylaminopurine (BAP) in combination with 2,4-D - naphtoxy acetic acid (2,4-D). Histological analysis showed the structure of Sinapis stigma (composed from four types of tissue: papillae, transmitting tissue, parenchyma and vascular bundles) and revealed that numerous meristematic centers developed from parenchyma cells in close vicinity of vascular bundles. Buds very quickly appeared on the surface of initial explants and later formed multiplantlets that were easily rooted in the soil.

5

Behaviour of stigma before and after pollination in olive tree (Olea europaea L.)

84%

Suarez C., Serrano I., Rapoport H. F., Olmedilla A., Rodriguez-Garcia M. I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

6

Test tube pollination of stigmas, ovaries and ovules of Salix viminalis with pollen grains of Alnus, Betula and Corylus

84%

Piosik L., Cieslak A., Zenkteler M., Zenkteler E.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

7

Micromorphology of glandular structures in Echium vulgare L. flowers

84%

Weryszko-Chmielewska E., Chwil M.

Acta Agrobotanica

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2008

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tom 61

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nr 2

The micromorphology of selected elements of Echium vulgare L. fl owers was investigated, with special attention to the structure of the nectaries and the stigma of the pistil as well as types of trichomes occurring on the surface of the calyx. The nectary had the shape of an uneven disc located around the lower region of the four-parted ovary of the pistil. The glandular cells formed a tier with a height of 330 μm and a radial width of 144 μm. Nectar was secreted onto the nectary surface through anomocytic stomata located at the level of other epidermal cells. Most of the stomata were open, with a different dimension of the pore. Their largest number was observed at the base of the nectary, and 462 stomata were noted on the whole surface of the nectary. The cuticle on the surface of the guard cells formed fi ne, circular striae. The subsidiary cells formed striated cuticular ornamentation, with the striae arranged radially in the direction of the stoma, whereas on the surface of other epidermal cells the striae formed an arrangement with different directions. The epidermis on the surface of the stigma formed regularly arranged papillae with a fan-shaped, expanded upper part which had corrugated outer walls, whereas the base of the cell formed a widened small column. The epidermis of the abaxial part of the calyx was covered by numerous non-glandular trichomes of different length which were made up of one or several cells. The glandular trichomes in the epidermis of the calyx grew with smaller density compared to the protective trichomes, and they were composed of a 1-2-celled stalk and a glandular head.

8

Effects of interaction between pollen coat eluates and pistil at the molecular level in self-compatible and self-incompatible plants of Lolium multiflorum Lam.

84%

Kalinowski A., Radlowski M., Bocian A.

Journal of Applied Genetics

|

2006

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tom 47

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nr 4

Two-dimensional electrophoresis (2-DE) of soluble proteins and enzymes was performed and specific activities of 5 enzymes (esterase, pectinesterase, acid phosphatase, protease and diaphorase) were determined in stigmas of Lolium multiflorum (Italian ryegrass) treated with self or foreign pollen coat eluates (pc). Also, a low-molecular-weight fraction of the treated self-compatible (SC) and self-incompatible (SI) stigmas was analyzed by high-pressure liquid chromatography (HPLC). The treatment of stigmas with foreign pollen induced the loss of 42% of the control sample proteins in SC plants but only of 5.5% in SI plants. In contrast, the treatment of stigmas with foreign pollen induced the loss of 15% proteins in SC plants and of 29% in SI plants. Specific activities of esterase, pectinesterase and diaphorase were higher in SC than in SI stigmas. The 2-DE enzyme patterns indicated qualitative relationships between the presence of some isoforms of acid phosphatase or protease and the treatment with self or foreign pc in SC and SI stigmas. No changes were observed in HPLC profiles of the low-molecular-weight fraction from SC and SI stigmas treated or not with pc. The presented results revealed different reactions of SC and SI stigmas to the treatment with self or foreign pc. Further investigations may explain if any of the observed reactions represent specific reorientations in the style, facilitating cross- or self-pollination.

9

The structure of floral elements of Anchusa officinalis L. creating attractants for insects

67%

Chwil M., Weryszko-Chmielewska E.

Acta Agrobotanica

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2009

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tom 62

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nr 1

The present study involved the measurement of size and the micromorphology of the floral elements of Anchusa officinalis L. which are attractants for insects. The structure of the epidermis on the surface of the calyx, petals, throat scales, pistil and nectary were analysed using light and scanning electron microscopy (SEM). For light microscopy observations, semi-permanent slides were prepared, which were treated with Lugol’s iodine solution, Sudan III and fluoroglucine. The dark violet lobes of the corolla of Anchusa officinalis, with a velvety surface, and the throat scales, contrasting with them, belong to the most important optical attractants which lure insects from large distances. The dark pink colouring of the sepals additionally increases the attractiveness of the flowers. The epidermis covering the calyx formed different-sized non-glandular trichomes as well as glandular trichomes. The glandular trichomes were composed of a uni – or bicellular leg and a unicellular head. The colour of the corolla petals was determined by anthocyanins accumulated in the epidermal cells and in the more deeply situated parenchyma. The velvety surface was formed by the conical papillae, densely growing from the adaxial epidermis. The pink-violet throat scales with white hairs, covering the inlet to the tube of the corolla, were found at the inlet to the corolla throat. The longest trichomes on the surface of the scales were located in their lower and middle parts, whereas the shortest ones at their tips. The epidermis of the central part of the throat scales formed small papillae. The trichomes had thin cell walls, large vacuoles, numerous plastids and lipid droplets. The two-parted stigma of the pistil was covered by characteristic expanded outgrowths with wavy edges which performed the functions of structures facilitating the capture of pollen grains. As a result of the present study it was found that the structures affecting the attractiveness of the flowers, through various light effects within the corolla of Anchusa officinalis, include the papillae on the corolla surface, trichomes of the throat scales and the epidermal cells of the style. The trichomes of the scales can also be responsible for protecting pollen and nectar against rainfall.

10

The structure of secretory tissue of the stigma and septal nectaries as well as nectar secretion of flowers of Hosta fortunei Baker L. H. Bailey (Funkiaceae)

67%

Chwil M.

Acta Agrobotanica

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2009

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tom 62

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nr 1

A study of Hosta fortunei Baker L. H. Bailey (Funkiaceae) flowers was conducted in the years 2007 – 2008. The flower life span and flowering duration as well as the nectar production rate were determined. The structure of the tissues of the stigma and nectary was investigated using stereoscopic, light and scanning electron microscopy (SEM). The plants flowered over a period of five weeks. Flowers of H. fortunei lived two days, on the average. Developed a pistil with an elongated ovary terminating in a discal stigma. On the surface of the stigma, unicellular glandular trichomes grew densely, composed of a stalk with a length of 113 – 213 μm and a head which was characterised by a large diameter range of 54 – 96 μm. The cuticle on the apical surface of a part of the trichomes was smooth, whereas it was striated on the stalk. Their protoplast was characterised by dense cytoplasm and weak vacuolisation. In the head of the trichomes fatty substances were accumulated. Septal nectaries occurred in the ovary of the superior pistil. Nectar was exuded onto the surface through three openings, situated in the upper part of the ovary of the pistil. At these places, epidermal cells formed a smooth or slightly wrinkled cuticle. The outer walls of the cells of the epidermis covering the duct accumulating nectar were thick. The glandular tissue of the nectary was made up of 2 layers of different-shaped, thin-walled cells and a deeply stained protoplast. They contained dense cytoplasm and a large, frequently lobate nucleus. At the final stage of secretion, fine vacuoles were observed in the cytoplasm of the glandular cells. Nectar secretion was abundant. In its initial stage, secretion droplets, increasing during the activity of the glandular tissue, were observed on the epidermis surface around the nectar openings. The weight of nectar from 10 flowers was 92.41 mg. The sugar concentration in the nectar was within a range of 23% – 30%, with an average value of 26%. Sugar yield was 23.83 mg/from 10 flowers.

11

The structure of some floral elements and the nectar production rate of Polemonium caeruleum L.

67%

Chwil M.

Acta Agrobotanica

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2010

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tom 63

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nr 2

The present study, carried out in the period 2008 – 2009, covered some morphological and anatomical features of the flowers of Polemonium caeruleum L. and their nectar production rate in the climatic conditions of the Lublin region. Observations were made with stereoscopic, light and scanning electron microscopy. Nectar production in the flowers was determined using the pipette method. The flowers of Polemonium caeruleum develop a calyx covered by an epidermis with numerous non-glandular and glandular trichomes. The secretory trichomes are composed of a several-celled stalk and a four-celled head. At the boundary of the corolla tube and the lobes, there is white colouration with violet nectar guides. The epidermis in this region produces several-celled living trichomes that close the entry into the corolla tube, thereby protecting the nectar accumulated in it. These hairs are at the same time glistening colour attractants for insects. The tripartite stigma of the pistil is covered, from the adaxial side, by unicellular papillae with striated cuticular ornamentation, growing at high density. Around the ovary there is located a nectariferous disc, in the form of a free projection, which secrets nectar with sugar concentration of 29 – 52% and sugar weight ranging 1.1 – 1.8 mg/flower.

12

Polyploidization of stigmatic papillae in Triglochin maritimum L. [Juncaginaceae]

67%

Bohdanowicz J., Dabrowska D.

Acta Biologica Cracoviensia. Series Botanica

|

1997

|

tom 39

The cytological differentiation of the dry papillate stigma in Triglochin maritimum L. (Juncaginaceae) was studied. The polyploidization process started soon after the formation of unicellular stigmatic papillae. Later, huge, long papillae with single enlarged nuclei constituted the receptive surface of the maturing stigma. The nuclear DNA content of the polyploid papillae and of telophasic (2C) and prophasic (4C) cells of the ovule was measured cytophotometrically after Feulgen staining. Analysis of nuclear DNA content measurements permitted the degrees of ploidy reached by the papillae to be established. Nuclei with DNA content corresponding to levels of 4C, 8C, 16C, 32C and 64C were found in the mature stigma. The most common were nuclei with DNA content of 16C (29%) and 32C (24%). The absence of mitoses, rhythmical enlargement of the DNA content of the nuclei as well as their characteristic endochromocenters, pointed to endoreduplication as the mechanism of polyploidization of the stigmatic papillae.

13

Localization of pectins and Ca2plus ions in unpollinated and pollinated wet [Petunia hybrida Hort.] and dry [Haemanthus albiflos L.] stigma

67%

Bednarska E., Lenartowska M., Niekras L.

Folia Histochemica et Cytobiologica

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2005

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tom 43

|

nr 4

Ograniczanie wyników

Self-injuries in adolescents: social competence, emotional intelligence, and stigmatization

Proteins of stigma exudate.

Tapetal raphides of Tradescantia bracteata: the strategy of their transport from anther to stigma

Adventitious bud regeneration from the stigma of Sinapis alba L.

Behaviour of stigma before and after pollination in olive tree (Olea europaea L.)

Test tube pollination of stigmas, ovaries and ovules of Salix viminalis with pollen grains of Alnus, Betula and Corylus

Micromorphology of glandular structures in Echium vulgare L. flowers

Effects of interaction between pollen coat eluates and pistil at the molecular level in self-compatible and self-incompatible plants of Lolium multiflorum Lam.

The structure of floral elements of Anchusa officinalis L. creating attractants for insects

The structure of secretory tissue of the stigma and septal nectaries as well as nectar secretion of flowers of Hosta fortunei Baker L. H. Bailey (Funkiaceae)

The structure of some floral elements and the nectar production rate of Polemonium caeruleum L.

Polyploidization of stigmatic papillae in Triglochin maritimum L. [Juncaginaceae]

Localization of pectins and Ca2plus ions in unpollinated and pollinated wet [Petunia hybrida Hort.] and dry [Haemanthus albiflos L.] stigma