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The effect of gamma radiation with 0, 200, 250, 300, 350, 400, and 450 Gy intensities on the longevity, total number of eggs, and the percent of hatched eggs laid by irradiated females of Tetranychus urticae Koch (Tetranychidae) was evaluated. Two different groups (0–24 h old and 48–72 h old) of adult females were irradiated. The results showed that 350 and 300 Gy doses significantly reduced the longevity of the 0–24 h old females and the 48–72 h old females. The younger females were more tolerant at lower dose rates than the older females. There was a quadratic relationship between dose rates and young females, while it was linear in older females. The total number of eggs laid by females of both ages was significantly reduced with a linear trend by 250 Gy irradiation. The eggs laid by females of both the 0–24 h olds and the 48–72 h olds lost their hatchability when the dose rate was 350 Gy. It was concluded, that applying a dose rate of 320 Gy on one of the mates (male or female) before mating, or a 300 Gy on both of them, would be sufficient to cause sterility in adult mites.
16-chromosome forms of red clover (2n=14+2) were crossed to six Trifolium species with the chromosome number 2n=16. Hybrid plants were derived from the cross of a stable 16-chromosome red clover T. pratense with T. diffusum (2n=16). No seeds were obtained from reciprocal crosses. F₁ hybrid plants were morphologically more similar to T. diffusum, whereas their other characters, e.g. flower number per head, were intermediate between the species crossed. All F₁ hybrids had the chromosome number 2n=16. Meiosis proceeded with large irregularities. The average number of bivalents per cell was 3.32, and that of univalents - 9.98. Univalents exhibited a high stickiness and frequently formed "end-to-end" configurations and chains consisting of about a dozen of so chromosomes. Bivalents were straight. Lagging chromosomes and chromosome bridges were observed during AI; lagging chromosomes were also found during AII. After an equalizing division, tetrads and different from them microspore polyads were formed. 16-chromosome hybrids were male- and female-sterile. No hybrids were obtained from the stable 16-chromosome red clover T. pratense (n=7 chromosomes) crossed to the selected clover species (T. apertum Bobr., T. alexandrinum L. and others) with n=8 chromosomes.
Niemann-Pick type C1 (NPC) disease is an autosomal recessive neurodegenerative disorder. One feature of the mouse model of NPC1 is it's infertility. We have made transgenic mice which express the Npcl protein exclusively in fibrillary astrocytes, using the glial fibrillary acidic protein (GFAP) promoter. This selective expression of Npcl corrects sterility in GFAP-Npc1E, Npcl-/- mice. Counts of acidophils in the pituitary of GFAP-Npc1E,Npc1-/-- mice, as compared to Npc1-/- mice, and measurements of dopamine D2 receptor (DRD2) mRNA in the pituitary, suggest mechanisms for fertility enhancement. We conclude that the correction of sterility in GFAP-Npc1E, Npc1-/- mice is a result of restoring hypothalamic control of the pituitary.
Cupressus Linn., commonly known as ‘cypress', belongs to the family Cupressaceae. It consists of 20 species with a wide and discontinuous distribution in the Northern Hemisphere. In the present study, three species, namely C. torulosa Don., C. arizonica Greene and C. glabra Sudw., growing in the western Himalayan region of India at an altitude of 1760 m. have been studied. This is a preliminary attempt where embryology is used in estimating seed quality and in seed testing. Seed quality was analysed according to five (0-IV) seed classes based on the presence of the embryo and endosperm and their nature of development in the seed-cutting test. Seeds having neither embryo nor endosperm belong to class ‘0'. In class ‘I' seeds contain the endosperm but a shrivelled embryo or sometimes no embryo. Class ‘II' contains seeds with an embryo of half-length of the embryo cavity and class ‘III' seeds containing an embryo of at least three-quarter length of the embryo cavity. Seeds with completely developed full embryo were assigned to class ‘IV'. However a high percentage of seeds of classes 0-III present in seed lots from particular trees of a species decrease the quality of seeds. Similarly a high percentage of seeds of class ‘IV' improves the seed quality of a given tree selected as plus tree for germplasm collection.
Study on the sterilizing and lethal effects of Cobalt-60 gamma radiation on the larval and adult stages of rust red flour beetle Tribolium castaneum (Herbst.) was done at 0, 10, 30, 50, 70, 100, 150, 300 and 500 gray (Gy). Irradiation severely affected pupal period, pupal formation and survivability of larvae and adults. The developmental period of the pest was found to increase along with the increase in the radiation doses. The irradiation at 70 Gy was found to cause complete sterility in the insect.
In F₂, F₃ and F₄ generations of one hybrid line of Lupinus varius × L. digitatus, segregation into infertile and fertile plants in the ratio 3:1 was observed. Cytoembryological analyses showed that sterility was caused by irregularities in megasporocyte formation, in megasporogenesis and megagametogenesis. The following abnormalities lead to female sterility: no megasporogenic cells separated in nucelluses; in other nucelluses with megaspore mother cells, these cells underwent vacuolation and died before reduction division. In the megaspore mother cells, in which probably meiosis occurs, the megasporocyte division is irregular; a restitution nucleus is frequently formed after reduction division and such megasporocyte develops into an embryo sac. In 8-nucleate embryo sacs a change in the nucellus polarization was observed, while in rarely encountered embryo sacs the embryo cells underwent vacuolation and then died. Female sterility in the studied segregants is determined genetically, whereas sterilization of reproduction cells is a developing process throughout the period of sporocyte and female gametophyte formation.
Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
To study mitochondria and plastid involvement in plant development, particularly in sexual reproduction, we made use of the Arabidospis thaliana T-DNA insertion collection developed in Versailles. Mutants affected in the nuclear genes that encode proteins predicted to be targeted to organelles were identified using two complementary strategies. In the first (forward genetics), mutants chosen for their sterile or gametophytic lethal phenotype were screened for T-DNA insertion in genes encoding mitochondrial or plastid proteins after systematic sequencing of the Flanking Sequence Tag (FST). The second (reverse genetics) enabled us to identify other mutants using the following tools: systematic A. thaliana proteome analysis, bioinformatics software to predict the sub-cellular localization of putative proteins, and the FST sequencing program FlagDB. Preliminary results for the first set of 82 putative mutants are presented and discussed.
Celem pracy jest wykazanie obecności bakterii w materiale pobranym z cewników dializowanych owiec w formie badań wstępnych. Pięć permanentnych kateterów zostało założonych pięciu owcom w żyłę szyjną zewnętrzną. Materiał do badań w postaci heparyny o stężeniu 1250 j.m./ml pobierano ze światła cewnika co 10 dni na przestrzeni czasu, w którym odbywały się dializy. Przeprowadzono 30 analiz mikrobiologicznych w celu określenia ogólnej liczby bakterii, bakterii Echerichia coli oraz bakterii z grupy coli. W 50% prób wykazano obecność drobnoustrojów, z czego tylko w jednej stwierdzono obecność bakterii z grupy coli. Implantacja permanentnych cewników hemodializacyjnych wiąże się z ryzykiem wystąpienia infekcji wtórnych. Wyniki badań wskazują na możliwość wystąpienia zakażeń odcewnikowych i rozwinięcie ewentualnej bakteriemii u hemodializowanych zwierząt.
The characteristic features of higher plant mitochondrial genomes: size, structure, recombination activity and evolutionary dynamics, are reviewed with the emphasis on the mitochondrial DNA (mtDNA) of Phaseolus vulgaris. Among all examined eukaryotic organisms, higher plants were found to contain the largest and most complex mitochondrial genomes. The plant mtDNA structure in vivo and mechanisms of evolution are controversial. We present the currently accepted models and how these models correspond to mitochondrial genomes of several common bean lines.
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