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In July 2013, symptoms of stem rot were observed in the Dracaena sanderiana cuttings in greenhouses of Mahallat County, Markazi Province, Iran. The symptoms first appeared as severe wilting. Later, leaves became brown and necrotic. Symptoms on the cuttings were observed as rotted areas on the middle of the stems. The cortical tissues of the plants showed a distinct brown discoloration. Eventually, the infected plants died. The pathogen was isolated from Dracaena stems and identified as F. solani by a fragment of the translation elongation factor 1-alpha (EF-1α) gene. Fusarium solani was confirmed by a pathogenicity test, and the causal agent was re-isolated from infected D. sanderiana plants. To the best of our knowledge, this is the first report of stem rot caused by F. solani on the cuttings of D. sanderiana.
A survey was conducted in February of 2004 on the outbreak of stem rot and wilt disease of pepper at the Kitabawa/Danzakara and Ajiwa irrigation sites in Northern Nigeria. Laboratory investigations revealed that it was elicited by Phytophthora capsici Leon. The disease caused severe loss in yield and USD 1 700.00 to USD 3 200.00 loss in revenue/ha. The disease was probably further aggravated by the presence of Fusarium sp. as well as ecto- and endoparasitic nematodes. Reasons for outbreak were elucidated and solutions proffered
Phytophthora root and stem rot of soybean is a destructive disease of soybean in Iran. During 1998-2005, 142 isolates from soil and diseased soybean plants were collected and tested. Race identification was made possible by inoculating Rps differential soybean cultivars and lines. Of the 142 isolates tested, 110 isolates belonged to race 1 and 32 isolates belonged to race 3. Race 1 was dominant in soil and diseased plant samples. There was no variability in virulence of Phytophthora sojae between the areas surveyed.
Stem and root rot symptoms were noticed in two greenhouse farms on tomatoes planted in the 3rd decade of June, 2013. Diseased plants exposed suppressed growth and top wilting due to drastic decrease of water and poor transport of mineral salt. From diseased stem and root parts Phytophthora nicotianae var. nicotianae was isolated. The same species was also detected in cubs and stonewool mats. In laboratory experiments isolates of P. nicotianae var. nicotianae caused rotting of stem parts, leaf petioles, leaf blades and fruits regardless of tested cultivar. Necrosis spread even more than 8 mm per 24 hrs. Colonisation of tomato stem parts was observed at temperature range from 10 to 32.5°C as the optimum. In the greenhouse trials P. nicotianae var. nicotianae added to peat with infested stonewool caused wilting of 3-week-old tomato cultivar Admiro seedlings already after 3-day-growth and 5 days later most of plants wilted and died. The pathogen colonized stem base of seedlings on the length up to 30 mm.
Rhizoctonia solani was isolated from 1/4–2 year-old seedlings of Silver fir, European larch, Scots pine and only sporadically from Norway spruce with symptoms of stem and root rot and yellowing and browning of needles. The species occurred on at least half of analysed, diseased plants. In laboratory trials the species colonised all parts of seedlings of Silver fir, European larch, Scots pine, Norway spruce and also Common alder and birch. On inoculated leaves of alder and birch necrosis spread about twice quicker than on needles of coniferous plants. Isolates from Norway spruce and Scots pine colonised plant parts quicker than from Silver fir and European larch. In greenhouse trial all isolates caused necrosis of 4 coniferous species and alder seedlings.
Basal Stem Rot (BSR) disease caused by Ganoderma lucidum (Leys) Karst, is the most destructive disease and a major constraint in coconut production. Fifty five endophytic strains of bacteria were isolated from coconut roots of different regions. Among the isolates, EPC5 (Endophytes coconut), EPC8, EPC15, EPC29, EPC52 and Pfl (Plant growth promoting rhizobacteria) promoted the rice seedling growth in roll towel and pot culture method. EPC5 (Plant growth promoting endophytic bacteria), Pf1 and Trichoderma viride (Plant growth promoting fungus) effectively inhibited the G. lucidum growth in vitro. When bioagents along with farm yard manure (FYM) were heaped for different days interval the population was increased in twenty days both in sterilized and unsterilized conditions.
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