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Identification of the DNA binding element of the human ZNF300 protein

100%

Qiu H., Xue L., Gao L., Shao H., Wang D., Guo M., Li W.

Cellular and Molecular Biology Letters

2008

tom 13

nr 3

391-403

The human ZNF300 gene is a member of the KRAB/C2H2 zinc finger gene family, the members of which are known to be involved in various developmental and pathological processes. Here, we show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. The ZNF300 DNA binding site, C(t/a)GGGGG(c/g)G, was defined via a random oligonucleotide selection assay, and the DNA binding site was further confirmed by electrophoretic mobility shift assays. A potential ZNF300 binding site was found in the promoter region of the human IL-2Rβ gene. The results of electrophoretic mobility shift assays indicated that ZNF300 bound to the ZNF300 binding site in the IL-2Rβ promoter in vitro. Transient co-transfection assays showed that ZNF300 could activate the IL-2Rβ promoter, and that the activation was abrogated by the mutation of residues in the ZNF300 binding site. Identifying the DNA binding site and characterizing the transcriptional regulation property of ZNF300 would provide critical insights into its potential as a transcriptional regulator.

From wheat to Arabidopsis: discovery and analysis of novel egg cell specific genes

100%

Sprunck S., Bellmann B., Gebert M., Baumann U., Dresselhaus T.

Acta Biologica Cracoviensia. Series Botanica. Supplement

2005

tom 47

nr 1

RNA interference against Biot2, a novel mouse testis - specific gene, inhibits the growth of tumor cells

80%

Wang C. T., Zhang P., Wang Y.-S., Ruan X.-Z., Li Z.-Y., Peng F., Yang H.-S., Wei Y.-Q.

Cellular and Molecular Biology Letters

2009

tom 14

nr 3

363-376

Biot2 is a novel murine testis-specific gene that was first identified using the SEREX technique, and named by our laboratory. Using conventional RT-PCR and real time RT-PCR, we tested the expression profile of Biot2 in normal tissues and various murine tumor cell lines. Using RNA interference, we studied the biological function of Biot2 in tumorigenesis. We applied various types of growth assay, such as the in vitro MTT, colony-forming and BrdU incorporation assays, along with in vivo tumorigenicity assays, to reveal its inhibition of tumor cell proliferation. The results revealed that the Biot2 transcript was detected only and strongly in the testis tissues and abundantly in five types of murine cancer cell line. Treating B16 murine melanoma, LL/2 murine Lewis lung carcinoma and CT26 murine colorectal adenocarcinoma with special shRNA targeting Biot2 can significantly reduce the proliferation rate of these three tumor cell lines in vitro, as measured by the MTT, colony-forming and BrdU incorporation assays. The tumorigenicity of the CT26 cells transfected with special shRNA targeting Biot2 was also decreased distinctly in vivo compared with the control. It was therefore concluded that Biot2 plays a key role in tumorigenesis and could be a potential target for biotherapy.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Baumann U.

1 Bellmann B.

1 Dresselhaus T.

1 Gao L.

1 Gebert M.

1 Guo M.

1 Li W.

1 Li Z.-Y.

1 Peng F.

1 Qiu H.

1 Ruan X.-Z.

1 Shao H.

1 Sprunck S.

1 Wang C. T.

1 Wang D.

1 Wang Y.-S.

1 Wei Y.-Q.

1 Xue L.

1 Yang H.-S.

1 Zhang P.

1 2009

1 2008

1 2005

Wyniki wyszukiwania

Identification of the DNA binding element of the human ZNF300 protein

From wheat to Arabidopsis: discovery and analysis of novel egg cell specific genes

RNA interference against Biot2, a novel mouse testis - specific gene, inhibits the growth of tumor cells