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Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos

100%
Wawrzyniak M., Hazubska-Przybyl T., Obarska A., Bojarczuk K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
2
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Tree somatic embryogenesis in science and forestry

100%
Hazubska-Przybyl T., Bojarczuk K.
Dendrobiology
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2016
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tom 76
Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
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Conifer somatic embryogenesis: II. Applications

100%
Cyr D. R., Klimaszewska K.
Dendrobiology
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2002
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tom 48
Somatic embryogenesis (SE) of conifers for clonal propagation has emerged from its earliest beginnings in 1980 to become an integral component of tree improvement strategies. With its capacity for long-term germplasm preservation and scale-up technologies,it is seen to be the preferred avenue to accelerate selection and operational deployment of value-added genotypes. At present,programs for numerous species from the Pinaceae family are underway worldwide,with activities ranging from selection trials to pilot production for plantation forestry. This paper will provide a review of current efforts in conifer SE including cryopreservation,commercialization and deployment strategies,and transgenics. A discussion of challenges and issues is directed at genetic fidelity, intellectual property and future needs.
4
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Conifer somatic embryogenesis: I. Development

80%
Klimaszewska K., Cyr D. R.
Dendrobiology
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2002
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tom 48
The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed.
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