Wyniki wyszukiwania

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Characteristics of the interspecific somatic hybrids Solanum pinnatisectum [plus] S. tuberosum H-8105

100%

Szczerbakowa A., Boltowicz D., Lebecka R., Radomski P., Wielgat B.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 3A

The morphological, cyto logical and molecular analyses of the plants regenerated after PEG-induced fusion between mesophyll protoplasts from the dihaploid potato clone H-8105 and the wild tuberous disease-resistant species S.pinnatisectum, were performed. A single fusion experiment yielded 313 calli, although only two calli produced shoots. From the rooted shoots, two stable clones (PT-01-1 and PT-01-2) exhibiting different vigor and habitat, were developed. The plants of PT-01-1 clone grew slowly in vitro, produced tubers after transfer to soil but did not set flowers. In contrast, the plants of the vigorous clone PT-01-2 produced both tubers and flowers after transfer to soil. The flower and tuber morphology of PT-01-1 and PT-01-2 regenerants was intermediate in comparison to the parental species. Cyto logical analysis revealed that the PT-01-1 clone was pentaploid and the PT-01-2 clone was tetraploid. The molecular (RAPD) analysis confirmed hybridity of both clones. The preliminary tests on late blight resistance of the hybrids showed no differences with a potato parent.

2

Plant protoplast technology: current status

100%

Davey M. R., Anthony P., Power J. B., Lowe K. C.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

Robust and reproducible protoplast-to-plant systems are crucial for underpinning genetic manipulation technology involving somatic hybridisation and transformation. Novel and effective approaches for maximising the efficiency of such protoplast cultures include supplementation of media with surfactants and artificial gas carriers, such as perfluorochemicals and haemoglobin. Physical parameters, particularly electrostimulation, also enhance the development of protoplasts and protoplast-derived cells in cullure. DNA uplake into protoplasts is now a routine and universally accepted procedure in plant biotechnology for introducing and evaluating both short-term (transient) and long-term (stable) expression of genes in cells and regenerated plants. Imporlantly, protoplast fusion overcomes pre- and post-zygotic sexual incompatibility barriers and generates novel germplasm through new nuclear-cytoplasmic combinations. In this respect, considerable progress has been made in generating somatic hybrid plants, particularly in citrus, brassicas and polato. Isolated protoplasts are also a unique single cell syslem for evaluating aspects of ultrastructure, genetics and physiology, with potential for the biosynthesis of novel secondary products, including commercially-important recombinant proteins (e.g. antibodies), and as systems in toxicity screening. Recent advances in protoplast technology have benefited from advances in animal and microbial cell culture, with interesting parallels existing between these systems. Further innovations will necessitate the strengthening of interdisciplinary links in these research fields and the requirement for continued dialogue and cooperation between workers with diverse but complementary skills.

3

Somatic hybridization between Solanum nigrum and Solanum tuberosum

100%

Szczerbakowa A., Maciejewska U., Borkowska M., Wielgat B.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

4

Miedzygatunkowe mieszance somatyczne ziemniaka

80%

Szczerbakowska A.

Ziemniak Polski

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2008

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tom 18

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nr 1

4-8

5

Use of somatic hybridisation to transfer resistance to late blight and potato virus Y [PVY] into cultivated potato

80%

Thieme R., Darsow U., Rakosy-Tican L., Kang Z., Gavrilenko T., Antonova O., Heimbach U., Thieme T.

Plant Breeding and Seed Science

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2004

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tom 50

6

Regeneration of leaf mesophyll protoplasts from Lycopersicon glandulosum, L. peruvianum and L. esculentum Stevens x Rodade hybrid

80%

Pindel A., Lech M., Miczynski K.

Acta Biologica Cracoviensia. Series Botanica

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1998

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tom 40

Protoplasts were isolated from mesophyll leaf tissue of in-vitro-grown 3-4-week-old seedlings of two wild tomato species L. glandulosum C.H. Mull. PI 731163 and L. peruvianum (L.) Mill. PI 128650, and from F4 plants of the Stevens x Rodade hybrid of L. esculentum Mill. For protoplasts isolation from both wild species a mixture of 0.3% cellulase + 0.03% macerozyme + 0.01% MES and 7.0% sorbitol was used, whereas maceration of leaf pieces from Stevens x Rodade was performed in a mixture of 0.3—1.0% cellulase + 0.2% macerozyme + 0.01% MES + 0.05% glycine and 7.0% sorbitol. Protoplast culture was carried out in a slightly modified nutrient medium of Tan et. al. (1987b). The yield of protoplasts per 1 g tissue amounted to 9 x 107 for both wild species, and 4 x 108 for the Stevens x Rodade plants. The first cell divisions of protoplasts from all three studied species occurred after 6-10 days of culture. Regenerated shoots rooted in 90% of both wild Lycopersicon species. Regeneration of Stevens x Rodade did not pass the greening callus stage.

7

Gentiana heterokaryons production with application of ECM 2001[BTX] pulse generator

80%

Fiuk A., Rybczynski J. J.

Biological Letters

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2005

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tom 42

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nr 2 Spec.Vol.

8

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Cytogenetic markers of Brassica napus L. chromosomes

70%

Hasterok R., Maluszynska J.

Journal of Applied Genetics

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2000

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tom 41

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nr 1

9

Domestication and genetics of the yellow lupin [Lupinus luteus L.] and the biotechnological improvement of lupins

61%

Swiecicki W., Rybczynski J., Swiecicki W. K.

Journal of Applied Genetics

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2000

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tom 41

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nr 1

Ograniczanie wyników

Characteristics of the interspecific somatic hybrids Solanum pinnatisectum [plus] S. tuberosum H-8105

Plant protoplast technology: current status

Somatic hybridization between Solanum nigrum and Solanum tuberosum

Miedzygatunkowe mieszance somatyczne ziemniaka

Use of somatic hybridisation to transfer resistance to late blight and potato virus Y [PVY] into cultivated potato

Regeneration of leaf mesophyll protoplasts from Lycopersicon glandulosum, L. peruvianum and L. esculentum Stevens x Rodade hybrid

Gentiana heterokaryons production with application of ECM 2001[BTX] pulse generator

Cytogenetic markers of Brassica napus L. chromosomes

Domestication and genetics of the yellow lupin [Lupinus luteus L.] and the biotechnological improvement of lupins