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This paper presents the results of studies carried out to estimate the influence of crop rotation, with diversified cereal shares on the humic acid characteristics. The tested range covered humic acids separated from the following soil types: degraded chernozem developed from loess, Orthic Luvisol developed from boulder clay, Orthic Luvisol developed from loess. Samples"were taken from the soil arable layer being under many-year crop rotation with a differentiated share of cereals (50 and 100%). The results obtained show that soil use affected quality indices of humic acids. In the lessive soils under cereal monocultures, an increase of the atomic H:C ratio was observed in humic acids. This allows for a conclusion that the monoculture system causes an increase in the content of aliphatic structures in humic acid molecules or a decrease in the condensation degree of their nuclei. Moreover, a decrease in the optic density of humin solutions given in A465 values was found in the humic acids under cereal monocultures which proves a marked direction of changes. In the case of humic acids extracted from chernozem, an analysis of elemental composition did not show any clear differentiation in the crop rotation with a 50% cereal share and a monoculture of cereal plants. The analysis of optic properties indicates a growth of the optic density (value of the E4:E6 ratio decreases) in the variant with a cereal plant monoculture which may suggest growth in the humification of individual humic acids.
The objective of the study was the estimation of quality of organic matter of the humus horizon of soil fertilised every year, for ten years, with spent mushroom (Agaricus L.) substrate, as compared to a soil without such amendment. Fractionation of organic matter was performed, as well as quality analyses of humic acids and fractionation of organic nitrogen complexes. In the humus horizon of the soil fertilised with spent mushroom substrate lower acidity was noted, and higher levels of C, N, P and Ca. In the soil amended with the substrate greater accumulation of soil humus was noted (by nearly 50%), and especially of the fraction of humic acids, which had a positive impact on increasing the value of the ratio of humic to fulvic acids. The fertilisation with the substrate only slightly modified the quality parameters of humic acids. In the soil fertilised with the substrate a higher level of carbon and nitrogen was observed – related with the soluble forms of organic matter, as well as greater accumulation of nitrogen, mainly in a stable non-hydrolysing form.
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