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Regulation of renal NAplus, Kplus-ATPase and ouabain-sensitive Hplus, Kplus-ATPase by the cyclic AMP-protein kinase A signal transduction pathway

100%

Beltowski A., Marciniak A., Wojcicka G., Gorny D.

Acta Biochimica Polonica

2003

tom 50

nr 1

We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling pathway on renal Na+ ,K+ -ATPase and ouabain-sensitive H+ ,K+ -ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the abdominal aorta proximally to the renal arteries for infusion of the investigated substances. Na+ ,K+ -ATPase activity was measured in the presence of Sch 28080 to block ouabain-sensitive H+ ,K+ -ATPase and improve specificity of the assay. Dibutyryl-cyclic AMP (db-cAMP) administered at a dose of 10-7 mol/kg per min and 10 -6mol/kg per min increased Na+ ,K+ -ATPase activity in the renal cortex by 34% and 42%, respectively, and decreased it in the renal medulla by 30% and 44%, respectively. db-cAMP infused at 10- mol/kg per min increased the activity of cortical ouabain-sensitive H+,K+ -ATPase by 33%, and medullary ouabain-sensitive H+,K+ -ATPase by 30%. All the effects of db-cAMP were abolished by a specific inhibitor of protein kinase A, KT 5720. The stimulatory effect on ouabain-sensitive H+ ,K+ -ATPase and on cortical Na+ ,K+ -ATPase was also abolished by brefeldin A which inhibits the insertion of proteins into the plasma membranes, whereas the inhibitory effect on medullary Na+ ,K+ -ATPase was partially attenuated by 17-octadecynoic acid, an inhibitor of cytochrome P450-dependent arachidonate metabolism. We conclude that the cAMP-PKA pathway stimulates Na+ ,K+ -ATPase in the renal cortex as well as ouabain-sensitive H+ ,K+ -ATPase in the cortex and medulla by a mechanism requiring insertion of proteins into the plasma membrane. In contrast, medullary Na+ ,K+ -ATPase is inhibited by cAMP through a mechanism involving cytochrome P450-dependent arachidonate metabolites.

Myrtus communis improves cognitive impairment in renovascular hypertensive rats

80%

Cevikelli-Yakut Z.-A., Ertas B., Sen A., Koyuncuoglu T., Yegen B. C., Sener G.

Journal of Physiology and Pharmacology

2020

tom 71

nr 5

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology

1 Beltowski A.

1 Cevikelli-Yakut Z.-A.

1 Ertas B.

1 Gorny D.

1 Koyuncuoglu T.

1 Marciniak A.

1 Sen A.

1 Sener G.

1 Wojcicka G.

1 Yegen B. C.

1 2020

1 2003

Wyniki wyszukiwania

Regulation of renal NAplus, Kplus-ATPase and ouabain-sensitive Hplus, Kplus-ATPase by the cyclic AMP-protein kinase A signal transduction pathway

Myrtus communis improves cognitive impairment in renovascular hypertensive rats