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Aim of the study: The study was conducted to assess the molecular relationship of verotoxigenic E. coli (VTEC) isolated from cattle slaughtered in abattoirs in the eastern part of Poland. Materials and Methods: A total of 50 non-O157 VTEC strains isolated from bovine hides and the corresponding carcasses were tested with the pulsed field gel electrophoresis (PFGE) method. Results and discussion: The XbaI restriction analysis enabled the clustering of the isolates into 19 PFGE profiles. Seven of them contained more than two strains with two groups of 8 isolates each. Furthermore, three profiles grouped two isolates and the remaining nine PFGE types were represented by only one strain. The PFGE results were analyzed in relation to identified VTEC serotypes (O186:H16, O185:H7, O181:H49, O177:H25, O175:H21, O174:H2, O153:H25, O153:H2, O148:H8, O139:H19, O117:H4, O91:HNT, O84:H28, O36:H19, O21:H25, O2:H6, O2:H32, ONT:H34) and presence of their virulence genes. It was found that strains of the same PFGE type were usually of the same serotype and possessed the same pathogenic markers. The most numerous profile was represented by eight isolates: all of these strains were identified as O2:H32 serotype and had the same virulence genes – vtx2 and vtx2e. Furthermore, the majority of the PFGE profiles grouped the strains isolated during the same day. PFGE analysis revealed that among the isolates obtained from hides and the corresponding carcasses five pairs of strains had an identical molecular profile. The present study provided valuable information concerning the molecular characterization of VTEC isolated from cattle at the slaughter level. The results reflected a low genetic diversity among VTEC isolates tested and may suggest a common source of contamination within the abattoir.
Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in the years 2005–2008 was evaluated. The study draws its data from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2005 and 2008 amounts to 21.24%.
Two traditionally maintained, small herds from southern Poland, with 8 and 12 sows, respectively, were surveyed coprologically during 2006-2007. In one of the herds, while deworming a group of sows with levamisole, faecal samples were collected on Day -7, Day 0 (the day of treatment) and Day 10, in order to assess the therapeutic effect of the drug. Coprological investigation was performed also in 26 fatteners originating from other small farms and slaughtered in a local abattoir, with their intestines washed through for the presence of roundworms. In both herds examined, Ascaris suum and Oesophagostomum spp. were prevalent, whilst Trichuris suis appeared only very rarely. Mainly fatteners, replacement gilts and young sows were highly infected with A. suum. The roundworm occurrence in 2-3 week-old piglets, with the intensity of 300 eggs per gram of faeces (EPG), indicated the possibility of parasite transmission to offspring very early in age. The highest level of Oesophagostomum spp. infection was observed in sows, but weaners were also much affected. For the group of dewormed sows, the mean faecal egg count reduction (FECR) was estimated to be 77.1 - 80.4%, suggesting the presence of resistant nodular worms. A very high false-positive A. suum egg counts found in slaughtered animals (240 to 320 EPG) testified to a high contamination level of the environment of small piggeries, as well. Since the reciprocal transmission of parasites between pigs and poultry might occur, it implies that the flocks should be raised separately.
The aim of this study was to determine the prevalence of large gastrointestinal parasites in horses, with the special focus on botflies from family Gasterophilidae as well as tapeworm Anoplocephala magna previously reported in Poland. 254 digestive tracts of 66 foals, born in 2009, 67 yearlings born in 2008, and 121 older horses were obtained from a slaughter-house near Krakow in 2009 and dissected. The horses were bred in small farms and sold mainly for economic profits. The history of horse deworming was unknown. Larvae of Gasterophilus intestinalis were the most common and found in 10.6% of the examined horses. Parascaris equonim was collected form 7.9% of the horse population, with the highest intensity of infection in foals and yearlings. The Oxyuris equi pinworm and Anoplocephala perfoliata tapeworm were observed only in two horses (0.8%), respectively.
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