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1

Sonic hedgehog pathway dysregulation in skin basal-cell carcinoma of a Polish population

100%
Lesiak A., Sobolewska-Sztychny D., Danilewicz M., Rogowski-Tylman M., Sysa-Jedrzejowska A., Sobjanek M., Olejniczak-Staruch I., Narbutt J.
Folia Histochemica et Cytobiologica
|
2013
|
tom 51
|
nr 3
2

Canine skin tumours with adnexal differentiation: histopathology and immunohistochemistry

100%
Jasik A., Kozaczynski W., Reichert M.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 2
277-283
Thirty nine canine adnexal tumours were histologically and immunohistochemically analysed. They were classified as trichoblastomas, trichoepitheliomas, sebaceous adenomas and epitheliomas, hepatoid gland adenomas and epitheliomas, and aporcine adenocarcinomas. Moreover, hepatoid gland angioadenoma and angioepithelioma were recognised. Studies of follicular tumours revealed coexpression of cytokeratin MNF116 and LP34, but sebaceous and hepatoid gland tumours as well as apocrine adenocarcinomas showed differences in the expression of both cytokeratins. All the tumours were negative for vimentin except two hepatoid gland adenomas in which coexpression of vimentin and cytokeratin was observed. Positive reaction for neuron-specific enolase (NSE) was observed in follicular tumours, whereas expression of α-smooth muscle actin (α-SMA) was found only in apocrine adenocarcinomas. Moreover, the presence of NSE observed in hepatoid gland adenomas was considered as non-specific just as both glial fibrillary acidic protein in sebaceous gland tumours and hepatoid gland adenomas, and also S100 protein in sebaceous adenomas. All the examined tumours showed lack of expression of both desmin and factor FVIII. Thus, among the used antibodies, cytokeratins, vimentin, NSE, and α-SMA play a main role in the evaluation of canine adnexal histogenesis.
3

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

80%
Kuzbicki L., Lange D., Stanek-Widera A., Chwirot B. W.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 3
4

Copper and zinc concentration in skin neoplastic tissues in dogs

80%
Brodzki A.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
Zinc and copper concentrations were assessed in skin tumours and serum of 26 dogs of different breeds. The analysed tumours were situated on legs, chest, and abdomen, 8, 7, and 5 cases, respectively. In 2 cases, the tumours were situated on the tail, anal region, and head. On the basis of a histopathological examination, 17 tumours were classified as malignant, while 9 were classified as benign ones. The dogs age-matched to those with neoplastic changes belonged to the control group. However, they were subjected to surgery due to reasons other than tumours. Samples of skin with subcutaneous tissue and serum obtained from these animals were handled in an identical procedure to those of the other groups. The spectrophotometric atomic absorption method and PAY-UNICAM apparatus were used to determine zinc and copper concentrations. It was demonstrated that the concentration of zinc and copper in malignant tumours was significantly increased in relation to the tissues obtained from healthy animals. Furthermore, tissue concentration of copper in benign tumours was significantly increased in comparison to the controls. In conclusion, the determination of zinc and copper concentrations in malignant neoplastic tissues and serum of dogs may be used as an additional diagnostic indicator conforming histopathological examination of tumour malignancy.
5

Metastasis of mammary carcinoma to myocardium in a dog: clinical and morphological correlation

80%
Nowak M., Noszczyk-Nowak A., Slawuta P., Nowaczyk R., Polanska J.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
A case of a widespread neoplastic process, originating from the mammary gland in mongrel bitch, weighing 27 kg and aging 7 years, was described. In the patient, blood morphology, blood biochemical parameters, gasometry of arterial blood, chest X-ray, and heart ECG and USG were determined. ECG record demonstrated elevation of ST-T segment by 0.2-0.3 mV in I, aVL leads and a downward- sloping depression in III and aVR leads. The alterations pointed with high probability to infarction in the lateral wall of the left ventricle. Following demonstration by imaging tests of numerous tumours in the chest, the dog was subjected to euthanasia. Upon autopsy, apart from the primary tumour in the mammary gland, numerous tumours were demonstrated in inner organs, including myocardium. Histopathology confirmed neoplastic growth of adenocarcinoma type. In the vicinity of the tumour localised in the left cardiac ventricle, microscopic examination disclosed regions of cardiomyocyte necrosis, which corroborated the preliminary diagnosis of infarction.
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