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Dehydroepiandrosterone protects against acetaminophen-induced liver damage in rats by upregulation of Bcl-2 and activation of SIRT signaling

100%

Abd-Ella E. M., El-Kott A. F., El-Kenawy A. E., Khalifa H. S., Bin-Meferij M. M., Alramlawy A. M.

Journal of Physiology and Pharmacology

2020

tom 71

nr 6

Sirtuin inhibition increases the rate of non-homologous end-joining of DNA double strands breaks

80%

Wojewodzka M., Kruszewski M., Buraczewska I., Xu W., Massuda E., Zhang J., Szumiel I.

Acta Biochimica Polonica

2007

tom 54

nr 1

Sirtuins (type III histone deacetylases) are an important member of a group of enzymes that modify chromatin conformation. We investigated the role of sirtuin inhibitor, GPI 19015, in double strand break (DSB) repair in CHO-K1 wt and xrs-6 mutant cells. The latter is defective in DNA-dependent protein kinase (DNA-PK)-mediated non-homologous end-joining (D-NHEJ). DSB were estimated by the neutral comet assay and histone γH2AX foci formation. We observed a weaker effect of GPI 19015 treatment on the repair kinetics in CHO wt cells than in xrs6. In the latter cells the increase in DNA repair rate was most pronounced in G1 phase and practically absent in S and G2 cell cycle phases. The decrease in the number of histone γH2AX foci was faster in xrs6 than in CHO-K1 cells. The altered repair rate did not affect survival of X-irradiated cells. Since in G1 xrs6 cells DNA-PK-dependent non-homologous end-joining, D-NHEJ, does not operate, these results indicate that inhibition of sirtuins modulates DNA-PK-independent (backup) non-homologous end-joining, B-NHEJ, to a greater extent than the other DSB repair system, D-NHEJ.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology

1 Abd-Ella E. M.

1 Alramlawy A. M.

1 Bin-Meferij M. M.

1 Buraczewska I.

1 El-Kenawy A. E.

1 El-Kott A. F.

1 Khalifa H. S.

1 Kruszewski M.

1 Massuda E.

1 Szumiel I.

1 Wojewodzka M.

1 Xu W.

1 Zhang J.

1 2020

1 2007

Wyniki wyszukiwania

Dehydroepiandrosterone protects against acetaminophen-induced liver damage in rats by upregulation of Bcl-2 and activation of SIRT signaling

Sirtuin inhibition increases the rate of non-homologous end-joining of DNA double strands breaks