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1

A simple method for the determination of the cholesterol esterase activity

100%
Stepien A. E., Gonchar M.
Acta Biochimica Polonica
|
2013
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tom 60
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nr 3
The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
2

A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

84%
Zhang S.-J., Wang L.-L., Lu S.-Y., Hu P., Li Y.-S., Zhang Y., Chang H.-Z., Zhai F.-F., Liu Z.-S., Li Z.-H., Ren H.-L.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 2
3
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A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

84%
Trzewik A., Nowak K. J., Orlikowska T.
Journal of Plant Protection Research
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2016
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tom 56
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nr 1
Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
4

Comparison of proteins based on segments structural similarity

84%
Plewczynski D., Pas J., Von Grotthuss M., Rychlewski L.
Acta Biochimica Polonica
|
2004
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tom 51
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nr 1
We present here a simple method for fast and accurate comparison of proteins us­ing their structures. The algorithm is based on structural alignment of segments of Ca chains (with size of 99 or 199 residues). The method is optimized in terms of speed and accuracy. We test it on 97 representative proteins with the similarity mea­sure based on the SCOP classification. We compare our algorithm with the LGscore2 automatic method. Our method has the same accuracy as the LGscore2 algorithm with much faster processing of the whole test set, which is promising. A second test is done using the ToolShop structure prediction evaluation program and shows that our tool is on average slightly less sensitive than the DALI server. Both algorithms give a similar number of correct models, however, the final alignment quality is better in the case of DALI. Our method was implemented under the name 3D-Hit as a web server at http://3dhit.bioinfo.pl/ free for academic use, with a weekly updated database containing a set of 5000 structures from the Protein Data Bank with non-homologous sequences.
5

Simple and efficient screening method for the detection of cephalosporin resistant Escherichia coli

67%
Wasyl D., Hoszowski A., Zajac M., Skarzynska M.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 2
147-151
An efficient method for cephalosporin resistance screening in E. coli isolated from healthy farm animals has been described. One hundred and twenty nine rectal swabs were streaked on MacConkey agar and selective medium supplemented with cefotaxime. Antimicrobial resistance was tested with broth microdilution and E. coli resistant to either/or cefotaxime and ceftazidime were further tested with Etest. The observed synergy of the compounds allowed confirming the presence of defined cephalosporin resistance phenotypes. The sensitivity of cephalosporin detection by the procedure with MacConkey culture reached merely 16.7% compared to the method with selective supplement medium. Extended spectrum of beta-lactamase producing isolates was found in strains isolated from 15 samples taken from turkeys, broilers, laying hens, and pigs. The ampC-type resistance was noted in E. coli from 33 samples originating from the same animal species. None of the resistance phenotypes was observed in cattle isolates. Attention is drawn to possible public health implications of slaughtered farm animals colonised with beta-lactam resistant E. coli.
6

Simple method to determine of sulfonamides in muscles by high performance liquid chromatography

67%
Pietruszka K., Posyniak A.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 3
A sensitive and selective LC method has been developed for the simultaneous determination of ten sulfonamides in muscles, after pre-column derivatization with fluorescamine. The whole procedure was validated in accordance with the Commission Decision 2002/657/EC. Detections capabilities (CCß) were from 125 µg/kg to 150 µg/kg, and recoveries ranged from 60% to 100% pending from analyte. The results demonstrate that the LC-FLD system is highly effective in analysing trace sulfonamides in muscles.
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