Wyniki wyszukiwania

1

Binding of harmane to human and bovine serum albumin: fluorescence and phosphorescence study

100%

Galecki K., Despotovic B., Galloway C., Ioannidis A.-G., Janani T., Nakamura Y., Oluyinka G.

Biotechnology and Food Science

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2012

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tom 76

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nr 1

2

Interaction of anesthetic supplement thiopental with human serum albumin

100%

Khan S. N., Islam B., Rajeswari M. R., Usmani H., Khan A. U.

Acta Biochimica Polonica

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2008

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tom 55

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nr 2

399-409

Thiopental (TPL) is a commonly used barbiturate anesthetic. Its binding with human serum albumin (HSA) was studied to explore the anesthetic-induced protein dysfunction. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. An increase in the binding affinity (K) and in the number of binding sites (n) with the increasing albumin concentration was observed. The interaction was conformation-dependent and the highest for the F isomer of HSA, which implicates its slow elimination. The mode of binding was characterized using various thermodynamic parameters. Domain II of HSA was found to possess a high affinity binding site for TPL. The effect of micro-metal ions on the binding affinity was also investigated. The molecular distance, r, between donor (HSA) and acceptor (TPL) was estimated by fluorescence resonance energy transfer (FRET). Correlation between the stability of the TPL-N and TPL-F complexes and drug distribution is discussed. The structural changes in the protein investigated by circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy reflect perturbation of the albumin molecule and provide an explanation for the heterogeneity of action of this anesthetic.

3

Expression of human serum albumin - L7-L12 [Brucella abortus ribosomal protein] fusion protein in Saccharomyces cerevisiae

100%

Pakzad I., Rezaee A., Emaneini M., Hosseini A. Z., Tabbaraee B., Taherikalani M.

Polish Journal of Microbiology

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2009

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tom 58

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nr 2

99-104

Brucella abortus is a facultative intracellular gram-negative bacterial pathogen that causes abortion in pregnant cattle and undulant fever in humans. The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. It has already been expressed in several bacteria and has been used as DNA vaccine. In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the 171112 ribosomal gene was amplified by PCR. The expression plasmid pYtHSA-L7/Ll 2 was constructed by inserting the L7/L12 gene into the pYHSA5 shuttle vector (containing inulinase signal sequence, HSA gene and Gal 10 promoter). The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L 12 antibodies. Fusion protein was purified by affinity chromatography and its amount was approximately 500 μg/liter.

4

Interaction of anthracene and its oxidative derivatives with human serum albumin

100%

Skupinska K., Zylm M., Misiewicz I., Kasprzycka-Guttman T.

Acta Biochimica Polonica

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2006

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tom 53

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nr 1

Binding affinities of ten polycyclic aromatic hydrocarbons to albumin were determined: anthracene, its eight oxy-derivatives: anthraquinone, 9-anthracenemethanol, 9-anthraldehyde, 9-anthracenecarboxylic acid, 1,4-dihydroxyanthraquinone, 1,5-dihydroxyanthraquinone, 1,8-dihydroxyanthraquinone, 2,6-dihydroxyanthraquinone and benzo[a]pyrene. The quenching of albumin fluorescence was used to measure the PAH — protein interaction. The theoretical curve of calculated fluorescence was fitted to experimental data after necessary corrections regarding PAHs fluorescence and inner filter effect. From the numerical fitting the final association constants were calculated. Anthracene and anthraquinone failed to quench the albumin fluorescence. 9-anthracenecarboxylic acid showed the highest, while 9-anthracenemethanol the weakest albumin binding affinity. The affinity constants determined for 9-anthraldehyde and benzo[a]pyrene were of the same magnitude and indicated low-affinity binding to albumin. The constants obtained for the four dihydroxyanthraquinones were higher, but dissimilar, which suggests that the position of the functional group in anthracene molecule influences the binding constant. Moreover, this study suggests that the type of substituent plays a significant role in PAH-albumin complex formation. The carboxylic group increases the binding affinity of the anthracene molecule the most rather than the presence of both carbonyl and hydroxyl groups. The lowest affinity constants were obtained for aldehyde, methyl and carbonyl substituents.

5

Studies on nongenomic action by steroid hormones

100%

Kotwicka M., Filipiak K., Butowska W., Warchol J. B.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

6

Interaction of alkylresorcinols with proteins

100%

Kozubek A.

Acta Biochimica Polonica

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1995

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tom 42

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nr 2

Alkylresorcinol homologs form stable monomolecular layers at air-water interface. Their interaction with proteins present in the subphase results in an increase of alkylresorcinol molecular packing in the extent dependent upon the protein studied. Strongest effects were observed for proteins with large hydrophobic regions, e.g. glycophorin or serum albumin. Interaction of proteins with alkylresorcinol monolayers is stronger than with phospholipids. A decrease and a shift of intrinsic protein fluorescence upon interaction with the compounds studied support their involvement in alteration of hydrophobic regions. For trypsin, 50% quenching was observed at the alkylresorcinol/trypsin ratio of 0.75. Concomitantly, an apparent inhibition of the enzymatic activity was noted. These results indicate that direct interaction of alkylresorcinols and modulation of enzymatic activities should be recognised as a significant part of the biological effect of these cereal bran components.

7

Immune regulation by sphingosine 1-phosphate and its receptors

80%

Bode C., Graler M. H.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

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nr 1

8

Influence of dendrimers on red blood cells

80%

Ziemba B., Matuszko G., Bryszewska M., Klajnert B.

Cellular and Molecular Biology Letters

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2012

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tom 17

|

nr 1

The emerging fields of tissue engineering and biomaterials have begun to provide potential treatment options for liver failure. The goal of the present study is to investigate the ability of a poly L-lactic acid (PLLA) nanofiber scaffold to support and enhance hepatic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs). A scaffold composed of poly L-lactic acid and collagen was fabricated by the electrospinning technique. After characterizing isolated hMSCs, they were seeded onto PLLA nanofiber scaffolds and induced to differentiate into a hepatocyte lineage. The mRNA levels and protein expression of several important hepatic genes were determined using RT-PCR, immunocytochemistry and ELISA. Flow cytometry revealed that the isolated bone marrow-derived stem cells were positive for hMSC-specific markers CD73, CD44, CD105 and CD166 and negative for hematopoietic markers CD34 and CD45. The differentiation of these stem cells into adipocytes and osteoblasts demonstrated their multipotency. Scanning electron microscopy showed adherence of cells in the nanofiber scaffold during differentiation towards hepatocytes. Our results showed that expression levels of liver-specific markers such as albumin, α-fetoprotein, and cytokeratins 8 and 18 were higher in differentiated cells on the nanofibers than when cultured on plates. Importantly, liver functioning serum proteins, albumin and α-1 antitrypsin were secreted into the culture medium at higher levels by the differentiated cells on the nanofibers than on the plates, demonstrating that our nanofibrous scaffolds promoted and enhanced hepatic differentiation under our culture conditions. Our results show that the engineered PLLA nanofibrous scaffold is a conducive matrix for the differentiation of MSCs into functional hepatocyte-like cells. This represents the first step for the use of this nanofibrous scaffold for culture and differentiation of stem cells that may be employed for tissue engineering and cell-based therapy applications.

9

Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose

80%

Sarabia-Sainz A., Ramos-Clamont G., Winzerling J., Vazquez-Moreno L.

Acta Biochimica Polonica

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2011

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tom 58

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nr 1

Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This interaction represents the first step in infection. Neoglycans referred to as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation of porcine serum albumin (PSA) with lactose at 80 °C, 100 °C and 120 ºC. Characterization studies of the products showed that PSA could contain 1, 38 or 41 added lactoses, depending on the reaction temperature. These neoglycans were approximately 10 times more glycated than PSA-Lac obtained in previous work. Lactose conjugation occurred only at lysines and PSA-Lac contained terminal galactoses as confirmed by Ricinus communis lectin recognition. Furthermore, Escherichia coli K88+, K88ab, K88ac and K88ad adhesins showed affinity toward all PSA-Lac neoglycans, and the most effective was the PSA-Lac obtained after 100 ºC treatment. In vitro, this neoglycan partially inhibited the adhesion of E. coli K88+ to piglet mucin (its natural ligand). These results provide support for the hypothesis that glycated proteins can be used as an alternative for bioactive compounds for disease prevention.

10

The efficiency of the replacement of protein meal by DDGS - dried distiller grains with solubles in the ration for dairy cows during early lactation

80%

Szulc T., Zachwieja A., Demkowicz M., Newlacil I., Pecka E.

Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry

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2011

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tom 14

|

nr 2

11

Level of serum albumin in crossbred pigs during the fattening period

80%

Kluczek S.

Annals of Animal Science. Supplement

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2002

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nr 2

12

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Effect of the combination of acetylsalicylic acid or chlormethine with antibiotics and glucose on the mammary gland of healthy cows

61%

Malinowski E.

Bulletin of the Veterinary Institute in Puławy

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1992

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tom 36

Ograniczanie wyników

Binding of harmane to human and bovine serum albumin: fluorescence and phosphorescence study

Interaction of anesthetic supplement thiopental with human serum albumin

Expression of human serum albumin - L7-L12 [Brucella abortus ribosomal protein] fusion protein in Saccharomyces cerevisiae

Interaction of anthracene and its oxidative derivatives with human serum albumin

Studies on nongenomic action by steroid hormones

Interaction of alkylresorcinols with proteins

Immune regulation by sphingosine 1-phosphate and its receptors

Influence of dendrimers on red blood cells

Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose

The efficiency of the replacement of protein meal by DDGS - dried distiller grains with solubles in the ration for dairy cows during early lactation

Level of serum albumin in crossbred pigs during the fattening period

Effect of the combination of acetylsalicylic acid or chlormethine with antibiotics and glucose on the mammary gland of healthy cows