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1

Comparison of two serotyping methods for Listeria monocytogenes

100%
Cabanova L., Cuvalova Z., Pipova M.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 4
Two methods used for serotyping of Listeria monocytogenes were compared: 1) in-house method, which is used routinely in the National Reference Laboratory (NRL), Dolný Kubín, (Slovakia) and 2) the method recommended by the European Union Reference Laboratory (EURL) in Paris. Thirty strains of L. monocytogenes used for serotyping were isolated from various food categories and swabs in both the EURL (10 isolates) and in the NRL (20 isolates). They showed the following serological profiles: 18 of them (60%) belonged to serogroup 1/2; seven strains (23%) belonged to serogroup 4, and five isolates (17%) were identified as serogroup 3. The most frequent L. monocytogenes serotypes in food were l/2a and 3a, whereas in swabs serogroup 4 was predominant. No differences in the detection of somatic O antigens were noticed among L. monocytogenes isolates between two methods of serotyping. In three isolates, however, some differences were found in the presence of flagellar H antigens, which were confirmed by the in-house method but were not revealed by the EURL method. In one isolate, the presence of flagellar HD antigen was not recovered by any of two serotyping methods tested. Based on the results of this study, the in-house method is more accurate, less laborious, and more convenient for routine diagnosis than the EURL method.
2

Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs

84%
Bancerz-Kisiel A., Szczerba-Turek A., Platt-Samoraj A., Szweda W.
Bulletin of the Veterinary Institute in Puławy
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2011
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tom 55
|
nr 1
The study has been taken up to collect more data on Yersinia enterocolitica isolated from pigs as the main reservoir and source of infection with strains pathogenic for humans. Bacteriological examinations, bio- and serotyping were conducted on 616 rectal swabs, taken from 308 fattening pigs. Two samples were taken from each animal to determine the ability of Y. enterocolitica to grow under different temperature conditions (warm and cold culture). It has been proven that low temperature constitutes a suitable culture condition. 138 Yersinia enterocolitica strains were isolated (22.40%), most of which (65.22%) were obtained in cold culture and 99.28% included in biotype 3 (one strain belonged to biotype 2). Serotyping yielded a positive result in 107 strains with the diagnostic serum for antigen O:3, in 18 - with the serum for antigen O:9, and 13 strains were determined to be non-typable. The results indicated that asymptomatic infections with Y. enterocolitica strains of the biotypes and serotypes pathogenic for humans are common in pig population.
3

Serotyping of Aeromonas species isolated from Polish fish farms in relation to species and virulence phenotype of the bacteria

67%
Kozinska A., Pekala A.
Bulletin of the Veterinary Institute in Puławy
|
2010
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tom 54
|
nr 3
The aim of the study was to determine the relationship between serogroups, species, and virulence of Aeromonas sp. Isolates from common carp and rainbow trout were tested for species designation and virulence phenotype and then serogrouped. A total of 558 isolates were tested. The bacteria were identified to species level using PCR-RFLP method. The ß-haemolysin, gelatinase, and caseinase activities were selected for virulence determination. The following species were dominant: A. hydrophila (35), A. bestiarum (103), A. salmonicida (98), A. sobria (101), A. veronii bt. sobria (171), and A. encheleia (30). 380 isolates were classified as virulent for fish. The isolates were serogrouped by agglutination tests according to the scheme of Sakazaki and Shimada. 478 isolates were serologically typeable (they did not show R type antigen or autoagglutination) and for 419 (87.6%) O-antigen was identified. The dominant serogroups among both carp and trout isolates were: O:11, O:16, O:18, O:33, PGO1, and PGO2. Groups O:3, O:6, O:41, PGO4, and PGO6 dominated among carp isolates and groups O:21, O:29, PGO5, and PGO9 were only represented by trout isolates. The relationship between Aeromonas serogroups and species was not found. Of the 15 dominant serogroups, eight groups included only isolates with virulence phenotype and two groups included only non-pathogenic isolates. The remaining groups were represented by virulent, as well as non-virulent isolates. Agglutination test can be used as alternative or complementary method to differentiate pathogenic and non-pathogenic isolates from carp and trout cultured in Poland.
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