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Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran

100%
Hajiyusef T., Shahraeen N., Maleki M.
Journal of Plant Protection Research
|
2017
|
tom 57
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nr 2
Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern provinces of Iran. During a survey in the 2015 growing season a total of 170 selected chickpea plants with general yellowing symptoms including stunting and leaf bronzing were collected. Serological Elisa and tissue blot immunoassay (TIBA) tests revealed the presence of Bean leaf roll virus (BLRV) and Chickpea chlorotic stunt virus (CpCSV) as the predominant viruses in the region. Some serologically positive samples of BLRV and CpCSV were selected and rechecked by RT-PCR. Th e results of amplifi ed PCR products using a specifi c pair of primers towards the Cp gene region of the viruses were approximately 413 bp for CpCSV and 391 bp for BLRV. Results obtained from sequence comparison of BLRV (IR-F-Lor-5) isolate form two subgroups with eight other BLRV isolates from GeneBank indicating a high homology of 96% with isolates from Argentina, Germany, Tunisia, USA, Spain, and Colombia. An isolate from Norabad (Iran) (IR-Nor) had 98% homology with HQ840727 Libyan isolate. CpCSV sequence comparison with six other GeneBank isolates indicated 98% homology with isolates from Tunisia and Azerbaijan. Th e overall results of this research revealed the CpCSV and BLRV (luteoviruses) associated with the yellowing disease syndrome of chickpea crops in the surveyed region.
2

Serological detection and variability of tomato yellow leaf curl virus isolates from Tanzania

100%
Kashina B. D., Mabagala R. B., Mpunami A. A.
Journal of Plant Protection Research
|
2007
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tom 47
|
nr 4
367-373
Tomato farms in Arusha, Morogoro, Dodoma, Iringa, Kilimanjaro and Coast regions of Tanzania were surveyed to assess the incidence of the yellow leaf curl disease, and to collect infected tomato leaf samples for sero-diagnosis. The triple antibody sandwich enzyme linked immunosorbent assay (TAS-ELISA) format was adopted for the detection of disease using commercial polyclonal antiserum and monoclonal antibodies SCRI 17, SCRI 20, SCRI 23 and SCRI 33. ELISA readings were rated on a scale of 0-4. The results of the tests indicated that all the Tomato yellow leaf curl virus (TY- LCV) isolates recorded high reaction values (4) with the polyclonal antibody. However, the Dodoma and Arusha isolates were rated highest in optical density (OD) reading with MAb SCRI 20 and 23. The remaining isolates produced lower OD values. All the isolates rated low (2) when tested with SCRI 33. The differences in reaction to the monoclonal antibodies of TYLCV indicated that variability exists between the coat protein epitopes of TYLCV and Tomato yellow leaf curl Tanzania virus (TYL-CTZV) on one hand, and among the TYLCTZV isolates on the other. Only the isolates from Arusha and Dodoma share a high sequence homology in coat protein with the European and related TYLCV isolates. Furthermore, the reaction with either SCRI 20 or SCRI 23 show that the isolates from Arusha and Dodoma share a high degree of homology, and could belong to one serotype. The other isolates from Morogoro, Coast and Kilimanjaro could form another serotype, while the isolate from Iringa is a different serotype. On the other hand, reaction with SCRI 17 groups the isolates in two serotypes, the Dodoma isolate alone, and another that groups the other five isolates together. It is recommended that other procedures such as DNA-DNA hybridization assays, polymerase chain reaction, restriction fragment length polymorphisms and sequencing can be combined with the use of monoclonal antisera for the detection and prediction or inference of Tomato yellow leaf curl disease (TYLCD) virus relationships at the quasi-species or strain levels in Tanzania.
3

Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis

100%
Hiszczynska-Sawicka E., Kur J., Pietkiewicz H., Holec L., Gasior A., Myjak P.
Acta Parasitologica
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2005
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tom 50
|
nr 3
The description of very efficient system for production and purification of Toxoplasma gondii recombinant antigens, GRA6, p35 and SAG2 is given in this study. The usefulness of these antigens for diagnostic of human infections was tested in an ELISA using 99 sera obtained during routine diagnostics. The sera for testing were selected from either acute or chronically infected patients. Both r-GRA6 and r-p35 antigens detected antibodies more frequently (p＜0.01) from acute (93.9 and 87.9%) rather than chronic (60.6 and 53.0%) infections. The r-SAG2 gave a similar sensitivity in both groups of patients (93.9 and 95.5%).
4

Importance of a single point mutation of the bovine leukemia virus [BLV] envelope glikoprotein in the serological detection of BLV infection

100%
Reichert M.
Bulletin of the Veterinary Institute in Puławy
|
2002
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tom 46
|
nr 1
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