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Lemon verbena is cultivated mainly due to the lemon-like aroma emitted from its leaves that are utilized for most purposes. The chemical composition of the essential oil of lemon verbena was analyzed by GC/MS in July at pre flowering, in September at full flowering and in end of October at post flowering period. The in vitro antimicrobial activity of the essential oil, extracted from Lippia citriodora was tested against laboratory control strains belonging to the American Type Culture Collection (Maryland, USA) four Gram (+) and Gram (-) bacteria using the disc diffusion test. Antioxidant activity of the sample was determined by 1,1-diphenil-2- -picrly-hydrazil (DPPH) assay. Well-known antioxidant compounds such as ascorbic acid and α-tocopherol were used as standard. Results showed, among different harvest periods high fresh and dry herbage and dry leaf yields per plant were obtained from full flowering stage. Harvest periods were found to have a significant effect on the content of essential oil and the highest amounts of limonene, neral and geranial and measured as 31.15, 11.92 and 15.53%, respectively at full flowering stage. In all samples, the main constituents were limonene, neral and geranial constituting 46.03%–58.59% of the total essential oil yield depend on development stages. Lemon verbena essential oil was found to have antibacterial activity especially against Gram (+) microorganisms. In DPPH system, the moderate radical scavenging activity was exhibited. Therefore, L. citriodora has potential to be used as a natural antioxidant and antimicrobial agent in food processing.
Salinity stress is a serious problem in urban landscape of arid and semi arid regions. To overcome the adverse impact of salinity, the application of organic matter and plant nutrients in the growth media for improving the plant growth is essential. An experiment was conducted in order to determine the response of Salvia splendens to salinity levels and also the role of humic acid in the salt stress alleviation. In the current experiment, five salinity levels (0, 20, 40, 60, and 80 mM NaCl) and three humic acid (0, 100, 500 and 1000 mg/l) treatments were prepared. The effects of these treatments were investigated on some growth parameters, physiological characteristics and also biochemical compounds. The results indicated that the growth parameters decreased in saline-treated than control plants. Different salinity levels significantly affected relative water content, evaporation rate and also electrolyte leakage. Salinity caused the increase in proline, malondialdehyde, sugar content, DPPH, total phenol content and decrease in chlorophyll, compare to the control plants. Application of humic acid on Salvia splendens decreased the leaf area and plant height compared to the control plants. Thus, regarding the growth parameters, it is probable that the effect of humic acid on the biochemical compounds is similar to salinity effect. The findings suggest that sage is an ornamental plant sensitive to salinity and humic acid (in the studied levels) could not alleviate the negative effects of salt stress on this plant.
The study aimed to determine agronomic characteristics and essential oil components of different basil ecotypes in semi-arid climatic conditions of South Eastern Anatolia, Diyarbakir, Turkey. Two-year harvest data about fresh and dry herb yield, dry leaf yield, essential oil content and its components from the plants of year 2015 and 2016 was analyzed in this study. Essential oil components were detected by gas chromatography/ mass spectrometry (GC/MS). The resulting outcomes demonstrated that both ecotypes and harvests had important effects on fresh and dry herb, dry leaf yield and essential oil contents of sweet basil. The highest dry leaf yield was noted from green leafy ecotypes and from second harvest stage. Twenty-three constituents were detected in the essential oil of O. basilicum ecotypes. The main components of basil essential oil were linalool, methyl chavicol, neral, geranial and methyl cinnamate that differed according to ecotypes and harvests during experimental years 2015 and 2016. Purple leafy basil ecotypes were determined as linalool rich, while greenish leaf ecotypes were abundant in methyl chavicol. Silbe – green ecotype contains higher neral and geranial levels than the other ecotypes. It was concluded that basil plant could be grown successfully and harvested two or more times to prefer for maximum dry leaf yield and essential oil contents under semi-arid climatic conditions.
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