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The aim of the work was to find how the abiotic stress factors, such as Zn and Ti ions, influenced on growth factor and accumulation of some elements in plantlets of C. asiatica (L.) Urban Apiaceae). Plantlets were cultured on Nitsch and Nitsch (NN) or Schenk and Hildebrandt (SH) modified media with various growth regulators. Zn ions as ZnSO₄ were additionaly put into medium in 4 concentrations for 3 to 7 weeks. Ti ions as 0.04% solution of Tytanit - foliar fertilizer were added into medium or stems of plantlets were soaking in solution only or with subsequent rinsing in sterile water. The concentrations of selected bioelements: Cu, Mn, Zn, Fe, Ca and Mg (determined by ASA using Spectrophotometer SP 1900 Pye Unicam in “free” (soluble in water) and “bound” (in cell structure) forms were evaluated The observations have shown that Zn and Ti application influenced on the weight and development of Centella asiatica cultured in vitro and caused changes in their mineral composition. Important differences in concentration of evaluated elements were found in studied samples. The content of analysed bioelements was dependent not only on Zn concentration in the medium but also of the form of Ti application or of the duration of the experiment.
The aim of this work was to analyze the concentrations of select elements such as sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn) in the water, muscle tissue, and gills of perch sampled in autumn from Lake Gopło in NW Poland. The correlations between the fish size (body length) and metal concentrations in the tissues were investigated by linear regression analysis. In addition, the bioaccumulation coefficient, as a measure of accumulation intensity of an element in an organ, was analyzed. The mean content of K, Mg, and Zn in the analyzed perch was higher in the muscle (18.90, 1.53, and 52.92, mg‧kg⁻¹) than in gills (8.88, 1.30, and 44.99 mg‧kg⁻¹), and the difference between these values (except for Zn) was statistically significant (p≤0.05). The analyses of the correlation between metal concentration in the meat and the body length of fish show that the bioaccumulation of Na, K, Mg, and Zn decreases as fish body length increases (negative correlation). The evaluation of the chemical pollution of Lake Gopło concentrations was based on the following ions (N-NO₃, N-NO₂, and P-PO₄) and minerals (Na, K, Ca, Mg, and Zn). The concentrations of nitrite nitrogen in the waters of Lake Gopło range from 0.012 (in September) to 0.057 mg N-NO₂ dm⁻³ (in November). The concentration of nitrate nitrogen ranges from 0.09 to 1.888 mgN-NO₃ dm⁻³. The concentration of orthophosphate in surface waters of Lake Gopło is not very diverse (0.17-0.2 mg PO₄⁻³ dm⁻³).
This paper provides information on the vertical distribution of cadmium and lead in the sediments, the amounts of the elements in the surface- and rain water, in selected fish organs, contents of their alimentary tracts, and in some plant species of the Świdwie Lake ecosystem. The levels and distribution of the analysed metals in the lake sediments gave evidence of their anthropogenic origin. The distribution of cadmium and lead in the organs and alimentary tracts of fishes, and especially the low level of the elements in fishes and plants suggests that the metals are linked to the abiotic components of the lake environment and they are not easily accessible for the organisms. The best bio-indicators of the pollution-level with cadmium and lead in the lake ecosystem are: the rudd, Scardinius erythrophtalmus L. and the roots of cattail, Typha angustifolia.
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