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Selected breeding characters and seed protein content of adzuki bean (Phaseolus angularis W.H. White) grown in Central Europe

100%

Gorna B., Szpakowska M., Nowak J., Holubowicz R.

Acta Agrophysica

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2016

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tom 23

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nr 4

In the years 2011-2012, thirteen cultivars and breeding lines of Chinese origin adzuki bean (Phaseolus angularis W.H. White) were evaluated for their selected breeding characters and seed protein content in the climatic conditions of Central Europe. In the field experiment, the evaluation included seedlings emergence, number of pods per plant, number of seeds per pod and plant health status. In the laboratory research, the assessments included seed germination as well as seed dry matter and total protein contents. In Western Poland climatic conditions, it was possible to produce seeds of adzuki bean. However, in cold and wet years, they did not set seeds. The tested materials differed in plant emergence, earliness, number of pods per plant, number of seeds per pod and plant health status. The produced seeds had germination capacity from 0 to 54% and no symptoms of bean weevil (Acanthoscelides obtectus Say) damage. They also had from 20.01 to 31.91% of total proteins in d. m., which proved they could be a valuable source of plant protein. Amongst the evaluated materials, one breeding line (marked with the letter J) turned out to be the best, whereas the other one (marked Z1) was also interesting for further breeding work.

2

Interval mapping of QTLs controlling yield-related traits and seed protein content in Pisum sativum

100%

Irzykowska L., Wolko B.

Journal of Applied Genetics

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2004

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tom 45

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nr 3

3

Geographic patterns of diversity of cultivated lentil germplasm collected from Pakistan, as assessed by seed protein assays

100%

Sultana T., Ghafoor A., Ashraf M.

Acta Biologica Cracoviensia. Series Botanica

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2006

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tom 48

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nr 1

Seed protein profiles were studied in 144 lentil accessions intensively collected from all over Pakistan. Heterogeneous populations were isolated on the basis of SDS-PAGE, and 13 polymorphic protein peptides were found, representing almost all the variation reported so far in lentil. The low diversity of accessions from the Northern Area and North Western Frontier Province, the most geographically diverse areas, suggested the need for more exploration so that the maximum genetic diversity of the areas can be truly represented. Clusters based on agro-ecological zones did not prove adequate for evaluation of lentil resources, whereas 63 of 108 accessions (58.3%) were grouped together by altitude and provincial distribution. The study confirmed the wealth of phenotypic divergence in the local lentil. A small sample of accessions from a particular region might not reflect the actual diversity within that region. Samples representing total diversity in particular countries or regions should be evaluated, so that a representative rather than a random set of accessions can be included in investigations of diversity on regional or continental scales. As Pakistan is in the vicinity of the centre of diversity of lentil, high variation of various parameters is expected, and that can be found only if a complete set of germplasm is studied.

4

Diversity of agronomic traits and total seed protein in black gram Vigna mungo [L.] Hepper

100%

Ghafoor A., Ahmad Z.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 2

This study evaluated agronomic traits of 111 genotypes of black gram Vigna mungo (L.) Hepper, mainly from Pakistan, to determine the extent of genetic diversity. Seed proteins were analyzed by SDS-PAGE. High genetic variance was observed for days to flowering, days to maturity, number of branches/plant, number of pods/plant, biomass/plant, grain yield/plant and harvest index, whereas low genetic variance was observed for pod length, seeds/pod and 100-seed weight in both years studied. SDS-PAGE of seed proteins showed low inter-accession diversity and no clear differentiation on the basis of origin or source. The genotypes in one cluster with similar agronomic characters did not necessarily belong to the same origin or source. The lack of a geographic pattern of germplasm collected from Pakistan was probably due to exchange of germplasm between neighbouring regions, or because germplasm represented progenies of the same ancestors. Clustering of advanced breeding lines along with an approved variety in one group revealed that only a portion of the genetic diversity has been exploited for improvement of black gram. It is suggested that the genetic base of cultivated black gram should be broadened by involving diverse parents in the breeding programme. Expansion of the genetic base for black gram breeding might be accomplished by systematic use of germplasm that differs in protein profiles and has better quantitative traits.

5

The amino acid composition of seed proteins and fractions of different lupine species

84%

Peretiatkowicz M., Wojtaszek P., Michalski Z., Gulewicz K.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 1-3

6

Comparative study of seed proteins in the genus Pisum. XV. Electrophoretic albumin patterns in P.sativum L. subsp. asiaticum Govorov

84%

Zimniak-Przybylska Z., Przybylska J., Gorecka D.

Genetica Polonica

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1992

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tom 33

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nr 2

7

Bioinformatics-aided characteristics of the structural motifs of selected potentially celiac-toxic proteins of cereals and leguminous plants

67%

Dziuba M., Dziuba J., Iwaniak A.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 4

405-414

A structural analysis was conducted of peptides responsible for inducing celiac disease, as well as of the structural fragments they are located in, i.e. the so-called “extended structural motifs”. These motifs originated from wheat A-gliadin and constituted a standard for the analysis of the other proteins. Experiments were carried out based on in silico methods. Analyses covered a total of 403 sequences of selected cereal and seed proteins. Of all the analysed sequences, 155 were found to contain tetrapeptides (potentially known as toxic for celiac disease), namely: QQQP, QQPY, PSQQ, QPYP, including 29 proteins in which the tetrapeptides were constituents of a structural motif with a longer sequence. These were proteins of wheat, barley and oat. All the extended motifs occurred in a hydrophilic surrounding, attaining the structure of beta-turn and random coil. Computer-aided design of the proteolysis process of selected proteins was carried out as well in the aspect of celiac-toxic peptide release. Active fragments were released from twenty-eight proteins by thermolysin, K proteinase and prolyl oligopeptidase.

8

Seed protein and esterase isozyme profile among the accession of lemongrass [Cymbopogon flexuosus (Nees ex Steud.) Wats] collected from environmentally different sites

67%

Sarma A., Sarma H., Handique G. K., Sarma T.C. T. C., Handique A. K.

Herba Polonica

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2011

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tom 57

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nr 3

Seed protein profile and esterase isoenzme were studied in ten accessions of lemongrass [Cymbopogon flexuosus] collected from northeastern India belonging to two major chemotypes – citral and geraniol rich essential oil. Total four esterase isozyme bands were ob55 Vol. 57 No. 3 2011 Seed protein and esterase isozyme profile among the accession of lemongrass served with Rm values in the range of 0.394 to 0.798. Among them one with Rm value 0.747 was unique as it was consistently present in all the accessions irrespective of citral or geraniol rich chemotype. For seed protein, SDS-PAGE analysis of seven accessions (RLJ-TC-1, RLJ-TC-4, RLJ-TC-5, RLJ-TC-8, RLJ-TC-9, RLJ-TC-10 and OD-19) revealed a total of 13 bands among the accessions ranging in the size from 21.5 to 92.0 kd. Six citral rich accessions exhibits very similar seed protein profile with 10 to 11 protein band each. However, the geraniol rich chemo-type RLJ-TC-8 exhibit different profile with only six high molecular proteins. Four seed proteins with molecular weight 92.0, 86.0, 80.4 and 61.6 kd were consistently found in all the chemotypes irrespective of citral or geraniol rich and can be considered as marker for the species. Although esterase isozymes exhibited low polymorphism, yet close similarity of isozyme and seed protein profile can be considered as evidence of genetic homogeneity among the accession of lemongrass.

9

Changes in plant water status, biochemical attributes and seed quality of black gram and green gram genotypes under drought

67%

Baroowa B., Gogoi N.

International Letters of Natural Sciences

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2015

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tom 42

Drought is one of the major abiotic stresses which adversely affect crop growth and production worldwide as water is vital for every aspect of plant growth and development. The present experiment was carried out during the growing seasons (September – December) of 2012 and 2013 to evaluate the response of black gram (Vigna mungo L.) and green gram (Vigna radiata L.) in terms of some important growth indices, biochemical traits and seed quality under drought stress. Four commonly grown genotypes - T9, KU 301(black gram) and Pratap, SG 21-5 (green gram) of Assam, India were grown in a randomized block design with three replications under stress and non-stress conditions. Stress was applied by withholding irrigation for fifteen consecutive days at vegetative, flowering and pod filling stages. Leaf area index (LAI), seed protein content and protein yield significantly decreased (p ≤ 0.01) whereas proline, total flavonoids and anthocyanin content increased significantly (p ≤ 0.01) in response to water deficiency. Among the studied genotypes, T9 and Pratap showed better tolerance capacity towards the applied drought by presenting higher values of LAI, plant height stress tolerance index (PHSI), dry matter stress tolerance index (DMSI), proline, total flavonoids, anthocyanin, lower percentage of chlorophyll degradation and finally producing high quality seeds.

10

Comparative study of seed proteins in the genus Pisum. XIV. Further evidence on the existence of electrophoretic albumin variant characteristic of P.fulvum Sibth. et Smith.

67%

Przybylska J., Zimniak-Przybylska Z., Gorecka D.

Genetica Polonica

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1992

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tom 33

|

nr 2

11

Screening of Pisum sativum L. germplasm against Erysiphe pisi Syd.

67%

Nisar M., Ghafoor A., Khan M. R., Qureshi A. S.

Acta Biologica Cracoviensia. Series Botanica

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2006

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tom 48

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nr 2

Powdery mildew (Erysiphe pisi Syd.) significantly reduces the yield and quality of pea all over the world. Screening of a broad range of germplasm revealed three highly resistant genotypes (Fallon, PS99102238 and PS0010128); eleven (Shawnee, Lifter, Franklin, PS610152, PS810240, PS710048, PS610324, PS810191, CGN3273, CGN3272, and PS9910188) showed symptoms after inoculation but the infection was not severe and recovery was rapid. Powdery mildew caused 86% loss to the germplasm, and the severity of the disease was associated with various phases. The pathogen inhibits seed development in the pod. Severe natural infection is expected to eliminate susceptible germplasm, some of which may have valuable, unique characteristics. The screening data were used to explore the relationship between susceptible and resistant genotypes, and between genetic diversity and geographic patterns. Seed protein assays did not sort genotypes by geographic pattern or disease resistance. It is suggested to transfer genes conferring disease resistance and economic yield to one genotype.

12

Comparative study of seed albumins in the genus Vicia

67%

Salmanowicz B. P., Krygier D.

Genetica Polonica

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1992

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tom 33

|

nr 1

13

Wplyw odmiany i terminu siewu na plon oraz sklad aminokwasowy bialka nasion lubinu zoltego

51%

Podlesny J., Strobel W.

Acta Agrophysica

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2007

|

tom 10

|

nr 1[151]

175-185

14

Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants

51%

Przybylska J.

Journal of Applied Genetics

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1995

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tom 36

|

nr 3

The article reviews the work of the author and coworkers on the use of electrophoretic protein analysis in taxonomie studies and classification of genetic resources of some leguminous plants: the genus Pisum, the Old-World Lupinus species and the cultivated Phaseolus species. On the example of the genus Pisum, the object of many years' investigations, the usefulness of electrophoretic analysis of different protein types - seed globulins, seed albumins and enzyme systems - is critically discussed. It has been concluded that variation in seed albumins, proteins often ignored in comparative analyses, should be more widely considered in taxonomie investigations. The conclusion was supported by the results obtained for Lupinus and Phaseolus species.

Ograniczanie wyników

Selected breeding characters and seed protein content of adzuki bean (Phaseolus angularis W.H. White) grown in Central Europe

Interval mapping of QTLs controlling yield-related traits and seed protein content in Pisum sativum

Geographic patterns of diversity of cultivated lentil germplasm collected from Pakistan, as assessed by seed protein assays

Diversity of agronomic traits and total seed protein in black gram Vigna mungo [L.] Hepper

The amino acid composition of seed proteins and fractions of different lupine species

Comparative study of seed proteins in the genus Pisum. XV. Electrophoretic albumin patterns in P.sativum L. subsp. asiaticum Govorov

Bioinformatics-aided characteristics of the structural motifs of selected potentially celiac-toxic proteins of cereals and leguminous plants

Seed protein and esterase isozyme profile among the accession of lemongrass [Cymbopogon flexuosus (Nees ex Steud.) Wats] collected from environmentally different sites

Changes in plant water status, biochemical attributes and seed quality of black gram and green gram genotypes under drought

Comparative study of seed proteins in the genus Pisum. XIV. Further evidence on the existence of electrophoretic albumin variant characteristic of P.fulvum Sibth. et Smith.

Screening of Pisum sativum L. germplasm against Erysiphe pisi Syd.

Comparative study of seed albumins in the genus Vicia

Wplyw odmiany i terminu siewu na plon oraz sklad aminokwasowy bialka nasion lubinu zoltego

Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants