The aim of the studies was to determine the effect of sawdust substrates and their enrichment with glucose on the mycelium growth and yield of three Hericium erinaceus (Bull. Fr.) Pers. strains. The subject of the studies was strains of H. erinaceus designated as ‘H1’, ‘D5’ and ‘D9’. Pine and beech sawdust supplemented with glucose in the amount of 1%, 2% and 3% were used as cultivated substrates. It was found that the tested strains differed in mycelium growth rate. The ‘D9’ strain was characterized by the fastest mycelium growth. The mycelium grew more rapidly on substrate with glucose addition, regardless from its amount, in comparison with substrate without glucose addition. The ‘H1’ and ‘D5’ strains gave the bigger yield than ‘D9’ one. The biggest yield was recorded on beech sawdust substrate with 3% addition of glucose.