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Molecular diagnostics of Sarcocystis spp. infections

100%

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.

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Eosinophilic myositis due to sarcocystosis in a herd of beef cattle

86%

Wouda W., Vos J. H., Snoep J. J., Sumter R. D.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

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Sarcosporidiosis: an emerging disease in yaks (Bos grunniens) on the Qinghai Tibetan Plateau (QTP), China

72%

Li K., Luo H., Mehmood K., Zhang H., Shahzad M., Abbas R. Z.

Acta Parasitologica

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2019

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tom 64

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nr 2

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Evaluation of an ELISA for the diagnosis of sarcocystosis in water buffaloes

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Ghorbanpoor M., Hamidinejat H., Nabavi L., Khadjeh G. H., Jalali M. R.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 2

To investigate the occurrence of sarcocystosis in water buffalo (Bubalus bubalis) in Ahvaz, the Khuzestan province, Iran, and to evaluate an ELISA for the diagnosis of sarcocystosis, serum and oesophagus samples were collected from the 300 water buffaloes, aged 0.5-7 years, and then slaughtered at the Ahvaz abattoir. The oesophagus samples were examined for sarcocysts under microscopic examination, using the digestion method. One hundred and seventy-one (57%) animals were found to be positive for Sarcocystis bradyzoites. One hundred and sixty-three (54.3%) serum samples were positive for sarcocystis antibodies in the ELISA. The prevalence of sarcocystosis was statistically age related, with significantly higher rates in adult buffaloes than young animals (P<0.05). The prevalence did not differ significantly in relation to the gender (P>0.05). The Mc Nemar test revealed a high correlation (94%) between the digestion method and ELISA. The ELISA, with the use of antigens from S. fusiformis bradyzoites, as presented in this study, can be adapted to detect antibodies to Sarcocystis sp., with an acceptable specificity and sensitivity.

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Protozoal macrocysts in the skeletal muscles of a mallard duck in Poland: the first recorded case

72%

Kalisinska E., Betlejewska K. M., Schmidt M., Gozdzicka-Jozefiak A., Tomczyk G.

Acta Parasitologica

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2003

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tom 48

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nr 1

Sarcocystosis of wild ducks, relatively common in North America, has hitherto been recorded only three times in Europe. The present study yielded a single case of macroscopically detected sarcocysts in the skeletal muscles of a mallard (Anas platyrhynchos). This finding constitutes the first such record in Poland. A PCR technique was used for identification of the parasite from sarcocysts. The results obtained suggest that the sarcocysts were produced by protozoans, which were the most closely related to Frenkelia glareoli and Sarcocystis neurona (homology of the nucleotide sequences was 96.6% in both cases).

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Sarcocystis spp. infection in free-living animals: wild boar (Sus scrofa L.), deer (Cervus elaphus L.), roe deer (Capreolus capreolus L.)

58%

Tropilo J., Katkiewicz M. T., Wisniewski J.