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Using of fresh slides and culture T. vaginalis was found in 1094 persons (3.20%). It has been seen the morhpological differentiation of T. vaginalis connected with the age, physiological state of macroorganisms and the clinic picture of trichomoniasis. In vivo, the spheroidal as well as ameboidal forms of T. vaginalis were observed. The latter ones, characteristic in acute trichomoniasis, were often seen in pregnant women. On the other hand, nonmobile round-shaped forms of T. vaginalis occurred in vaginal contents of girls, women in child-bed and old (5 years after menopause) women. It seems, that morphologicaly variability of T. vaginalis depends on changes of specific environment of human vagina, which is very sensitive to hormon responses. It may be considered as adaptation of parasite to different biological conditions of vaginal environment.
It has been shown that glycoprotein and polysaccharide fractions obtained from T. vaginalis cells can be employed as antigens in serodiagnosis of trichomonosis. This refers especially to the polysaccharide fraction because of its easy availability, solubility in aqueous solutions, high diagnostic specificity and sensitivity. It has been stated that the diagnostic value of polysaccharide antigen of T. vaginalis, in occult trichomonosis in men particularly, is higher than the effect of the secretion examination by means of culture, even after the prostatic gland massage.
During 5 years cycle a prophylactic gynaecological, cytological and parasitological examinations of 1918 women were performed. 12.8% of women were infected with T. vaginalis. Decreasing percentage of infections was observed: from 9.92% in 1984 to 2.2% in 1988. In the I° of Papanicolaou cervical smears T. vaginalis has been found in 1.60% of cases, in the II°-in 5.2%, in the III°- in 14.29%, and in the IV°-in 22.22% of cases. In women with normal cervix uteri T. vaginalis have been found in 5.01% of cases, and in women with hypertrophical cervix and partially epidermised ectopy with ovula Nabothi in 15.28% of cases.
Data concerning 604 girls up 18 year old were analysed. On the basis clinical symptoms and results of culture of various biological materials on selected media - the monofocal trichomonadosis concerning the most often vulva and vagina, rarely urethra or other regions of urinary organ as well as the multifocal trichomonadosis, particularly complicated by mycosis were proved. It was found the convergence statistically significant (P≤0,5) of the invasion of T. vaginalis with some symptoms of inflammation of genital and urinary organs; the highest values of Pearson-Bravais coefficient was obtained in trichomonadosis of genital organs and vaginal discharges, pain of vulva and vagina, the picture of colpitis maculosa, but in the multifocal one also with itching and pain in urethra and dysuria. T. vaginalis without symptoms occurred very seldom in the girls.
19 024 girls (aged: from 0 to 18) attending the Pediatrie Gynaecology Outpatient Department and the Center for Treatment of Parasitic and Mycotic Diseases were examined. The diagnosis of infection with T. vaginalis was based on preparations using various methods and cultures on specific media, from vaginal content and urine. It was found that the frequency of T. vaginalis invasions in girls had lessened 2-6 times in different groups from 1960 till 1985 and the higher percent of T. vaginalis in girls over 10 years old had been observed than in younger ones. There exists the statistically significant correlation (P < 0,02) between T. vaginalis invasions in girls and other persons from their environment (families).
The 50% trichomonocidal concentration of 7 examined ethers (T. vaginalis strain N° 1/86, Roiron medium, safranine staining, 30 min.) was estimated by means of dose-response curve. The minimal mycostatic concentration (Candida albicans L-45, Geotrichum candidum; 3% Sabouraud agar, 37°C, 24 hr) was calculated with the aid of regression equation. All examined ethers show in vitro a marked trichomonocidal effect comparable with activity of ornidazole or phenol. Mycostatic activity of new compounds is many times higher than activity of phenol, but considerably lower than that of klotrimazole. The strongest complex trichomonoddal and mycostatic effect-comparable to ornidazole or klotrimazole - shows the new derivative N,N-diethylaminoethyl oxime of 1-tioflavone (compound II), which is fairly toxic for mammal.
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