Wyniki wyszukiwania

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Localization of lead in root tip of Dianthus carthusianorum

100%

Baranowska-Morek A., Wierzbicka M.

Acta Biologica Cracoviensia. Series Botanica

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2004

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tom 46

45-56

The distribution of lead in root tips of Dianthus carthusianorum was compared in populations from a zinc-lead waste heap in Bolesław near Olkusz and from a natural stand in the Botanical Garden in Lublin. The analyses were made at two developmental stages: seedlings (after 8 days of incubation in 5 mg/dm3 Pb+2 from PbCl2 in 1/8 Knop medium) and mature plants (after 23 days of incubation in 10 mg/dm3 Pb+2 from PbCl2 in 1/2 Knop medium). Histochemical methods (rhodizonate and dithizonate) of lead detection revealed significant accumulation of this metal on the root surface of the examined plants. The site of next-strongest lead accumulation in root tips of plants from both populations was in cell walls of the pericycle. The layer of meristematic pericycle cells seemed to be a strong barrier against penetration of lead to the deepest cells of the procambium. Histochemical methods and tissue sections revealed no differences in lead distribution between root tips from the waste heap and from the natural population, but differences were detected on the ultrastructural level. There were numerous lead deposits in the cytoplasm of cells from ground meristem in the natural population, and none in specimens from the waste heap, indicating that lead had a higher toxic effect on the natural population of D. carthusianorum.

2

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Suitability of selected Polish field bean cultivars (Vicia faba var.minor) for the root tip genotoxicity assay (Vicia RTA)

100%

Obidoska G., Korzeniowska M., Hadam A.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2017

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tom 38

3

Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L.

100%

Zou J., Yue J., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2012

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tom 54

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nr 1

Allium cepa var. agrogarum L. seedlings grown in nutrient solution were subjected to increasing concentrations of Cd2+ (0, 1, 10, 100 μM). Variation in tolerance to cadmium toxicity was studied based on chromosome aberrations, nucleoli structure and reconstruction of root tip cells, Cd accumulation and mineral metabolism, lipid peroxidation, and changes in the antioxidative defense system (SOD, CAT, POD) in leaves and roots of the seedlings. Cd induced chromosome aberrations including C-mitoses, chromosome bridges, chromosome fragments and chromosome stickiness. Cd induced the production of some particles of argyrophilic proteins scattered in the nuclei and even extruded from the nucleoli into the cytoplasm after a high Cd concentration or prolonged Cd stress, and nucleolar reconstruction was inhibited. In Cd2+-treated Allium cepa var. agrogarum plants the metal was largely restricted to the roots; very little of it was transported to aerial parts. Adding Cd2+ to the nutrient solution affected mineral metabolism. For example, at 100 μM Cd it reduced the levels of Mn, Cu and Zn in roots, bulbs and leaves. Malondialdehyde content in roots and leaves increased with treatment time and increased concentration of Cd. Antioxidant enzymes appear to play a key role in resistance to Cd under stress conditions.

4

Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.

100%

Pang N., Zhang F.

Acta Biologica Cracoviensia. Series Botanica

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2015

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tom 57

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nr 1

In this study, wheat (Triticum aestivum L.) roots were treated with hypoxic water. The staining of cell preparations with DAPI revealed morphological changes of the cells such as nuclear condensation, deformation and fragmentation. Under TEM, cellular membrane shrinkage and breakage, chromatin condensation and apoptotic- like bodies were displayed. The number of mitochondria increased dramatically; their cristae were damaged; the interior became a cavitation and only some flocculent materials were distributed. Indirect immunofluorescence staining indicated that cytochrome C diffused from mitochondria to nucleoplasm and cytoplasm. TUNEL positive nuclei indicated double strand breaks of DNA. DAB staining was used for the identification of hydrogen peroxide and examination showed that the longer the treating time, the darker the staining of the meristematic zones of the roots which suggested the increased accumulation of these Reactive Oxygen Species (ROS). The elevation of hydrogen peroxide production was paralleled with the increase of SOD and POD activities. A negative correlation between the exposure time under hypoxia and the contents of soluble proteins was found. No obvious effect of hypoxia on MDA was established. The obtained results demonstrate that hypoxia causes programmed cell death in the root-tip meristematic cells of Triticum aestivum L. which is most probably attributed to the accumulation of large amounts of ROS.

5

The effect of lead on the ultrastructure changes in the root tip of onion - Allium cepa L.

84%

Wierzbicka M.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 4

6

Pb entrance and accumulation regions in Populus tremula x P. tremuloides and Arabidopsis thaliana roots

84%

Rabeda I., Basinska A., Glama M., Mellerowicz E. J., Napieralska A., Ratajczak M., Wozny A., Krzeslowska M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

7

Phytotoxicity and phytogenotoxicity of municipal sewage sludge

84%

Obidoska G., Karaczun Z., Zarska B.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2020

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nr 41

Ograniczanie wyników

Localization of lead in root tip of Dianthus carthusianorum

Suitability of selected Polish field bean cultivars (Vicia faba var.minor) for the root tip genotoxicity assay (Vicia RTA)

Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L.

Hypoxia-induced programmed cell death in root-tip meristematic cells of Triticum aestivum L.

The effect of lead on the ultrastructure changes in the root tip of onion - Allium cepa L.

Pb entrance and accumulation regions in Populus tremula x P. tremuloides and Arabidopsis thaliana roots

Phytotoxicity and phytogenotoxicity of municipal sewage sludge